Targetable Gold Nanorods for Epithelial Cancer Therapy Guided by Near-IR Absorption Imaging
Targetable Gold Nanorods for Epithelial Cancer Therapy Guided by Near-IR Absorption Imaging
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DOI：
10.1002/smll.201101789
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发表时间：
2012-03-12
期刊：
影响因子：
13.3
通讯作者：
Haam, Seungjoo
中科院分区：
文献类型：
--
作者：
Choi, Jihye;Yang, Jaemoon;Haam, Seungjoo
精心设计的纳米粒子介导的图像引导的癌症治疗已经引起了人们对提高癌症治疗功效的兴趣。提出了一类新的采用西妥昔单抗（CET）缀合的聚乙二醇（PEG）化的金纳米棒（CET-PGNRs）的智能诊断纳米探针;这些纳米探针使用近红外光靶向上皮癌细胞。GNR上的十六烷基三甲基溴化铵双层被异双功能PEG（COOH-PEG-SH）取代，以用作生物相容性稳定剂并增加特异性。使用1-乙基-3-（3-二甲基氨基丙基）碳二亚胺/N-羟基琥珀酰亚胺（EDC-NHS）化学用CET进一步官能化羧化GNR。为了评估这种GNRs的潜力，研究了它们的光学性质、生物相容性、胶体稳定性、对癌细胞的体外/体内结合亲和力、吸收成像和光热治疗效果。CET-PGNRs表现出优异的肿瘤靶向能力和同时吸收成像和光热消融上皮癌细胞的强大潜力。
Well-designed nanoparticle-mediated, image-guided cancer therapy has attracted interest for increasing the efficacy of cancer treatment. A new class of smart theragnostic nanoprobes employing cetuximab (CET)-conjugated polyethylene glycol (PEG)ylated gold nanorods (CET-PGNRs) is presented; these nanoprobes target epithelial cancer cells using near-infrared light. The cetyltrimethylammonium bromide bilayer on GNRs is replaced with heterobifunctional PEG (COOH-PEG-SH) to serve as a biocompatible stabilizer and to increase specificity. The carboxylated GNRs are further functionalized with CET using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC-NHS) chemistry. To assess the potential of such GNRs, their optical properties, biocompatibility, colloidal stability, in vitro/in vivo binding affinities for cancer cells, absorption imaging, and photothermal therapy effects are investigated. CET-PGNRs exhibit excellent tumor targeting ability and strong potential for simultaneous absorption imaging and photothermal ablation of epithelial cancer cells.