Destruction of the securin Pds1p occurs at the onset of anaphase during both meiotic divisions in yeast
Destruction of the securin Pds1p occurs at the onset of anaphase during both meiotic divisions in yeast
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DOI：
10.1007/s004120050409
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发表时间：
2000-04-01
期刊：
影响因子：
1.6
通讯作者：
Nasmyth, K
中科院分区：
文献类型：
--
作者：
Salah, SM;Nasmyth, K
姐妹染色单体的凝聚力是在DNA复制过程中建立的，它依赖于一种称为凝聚素的多蛋白复合物。在分裂后期开始时，将姐妹篇聚集在一起的内聚结构必须被破坏，以允许姐妹篇的隔离。在芽殖酵母酿酒酵母中，姐妹染色单体凝聚力的丧失取决于一种称为Esp1的分离蛋白（separin）。在中期到后期的过渡期，分离蛋白通过其抑制亚基（securin）（称为Pds1）的蛋白水解被激活。这一过程由后期促进复合物和辅助蛋白Cdc20介导。在减数分裂中，一轮DNA复制之后是两轮连续的分离。因此，凝聚力的丧失被分散到两个部门。通过研究启动减数分裂后期的机制，我们发现，酵母securin Pds1p存在于减数分裂的细胞核中，并在每次减数分裂开始时被破坏。我们还表明，securin破坏依赖于Cdc20p，它积累在细胞核内的时间左右的Pds IP的消失。
Sister chromatid cohesion is established during DNA replication and depends on a multiprotein complex called cohesin. At the onset of anaphase the cohesive structures that hold sisters together must be destroyed to allow segregation of sisters. In the budding yeast Saccharomyces cerevisiae loss of sister chromatid cohesion depends on a separating protein (separin) called Esp1. At the metaphase to anaphase transition, separin is activated by proteolysis of its inhibitory subunit (securin) called Pds1. This process is mediated by the anaphase promoting complex and an accessory protein Cdc20. In meiosis a single round of DNA replication is followed by two successive rounds of segregation. Thus loss of cohesion is spun out over two divisions. By studying the mechanisms that initiate anaphase in meiotic division we show that the yeast securin Pds1p is present in meiotic nuclei and is destroyed at the onset of each meiotic division. We also show that securin destruction depends on Cdc20p which accumulates within nuclei around the time of Pds Ip's disappearance.