Comparison of the Intraperitoneal, Retroorbital and per Oral Routes for F-18 FDG Administration as Effective Alternatives to Intravenous Administration in Mouse Tumor Models Using Small Animal PET/CT Studies
Comparison of the Intraperitoneal, Retroorbital and per Oral Routes for F-18 FDG Administration as Effective Alternatives to Intravenous Administration in Mouse Tumor Models Using Small Animal PET/CT Studies
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DOI：
10.1007/s13139-011-0087-7
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发表时间：
2011-09-01
影响因子：
1.3
通讯作者：
Kim, Seok-ki
中科院分区：
文献类型：
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作者：
Kim, Chulhan;Kim, In Hye;Kim, Seok-ki
目的比较F-18-氟脱氧葡萄糖（FDG）经眶后（RO）、腹腔内（IP）和口服（PO）途径与静脉内（IV）途径对小鼠正常组织和肿瘤的作用。当肿瘤生长到直径约9 mm时，在通过RO、IP、PO或IV途径给予FDG后进行正电子发射断层扫描（PET）。结果经RO、IP和IV途径给药后60 min，正常组织摄取FDG的量无显著性差异。然而，PO给药显示延迟分布和不必要的胃肠道高摄取。肿瘤摄取FDG表现出类似的时间模式，并增加，直到60分钟后，在RO，IP和IV注射组FDG管理。在PO给药组中，肿瘤摄取延迟并减少。RO、IP和IV给药组之间的额外系列PET扫描无统计学差异。结论RO给药是一种有效的替代途径，IV给药用于正常小鼠和肿瘤模型的小鼠FDG PET扫描。此外，IP给药可能是晚期的一种实用替代方案，尽管初始摄取低于IV和RO组。
Purpose We compared alternative routes for F-18-fluorodeoxyglucose (FDG) administration, such as the retroorbital (RO), intraperitoneal (IP) and per oral (PO) routes, with the intravenous (IV) route in normal tissues and tumors of mice.Materials and Methods CRL-1642 (ATCC, Lewis lung carcinoma) cells were inoculated in female BALB/c-nu/nu mice 6 to 10 weeks old. When the tumor grew to about 9 mm in diameter, positron emission tomography (PET) scans were performed after FDG administration via the RO, IP, PO or IV route. Additional serial PET scans were performed using the RO, IV or IP route alternatively from 5 to 29 days after the tumor cell injection.Results There was no significant difference in the FDG uptake in normal tissues at 60 min after FDG administration via RO, IP and IV routes. PO administration, however, showed delayed distribution and unwanted high gastrointestinal uptake. Tumoral uptake of FDG showed a similar temporal pattern and increased until 60 min after FDG administration in the RO, IP and IV injection groups. In the PO administration group, tumoral uptake was delayed and reduced. There was no statistical difference among the RO, IP and IVadministration groups for additional serial PET scans.Conclusion RO administration is an effective alternative route to IV administration for mouse FDG PET scans using normal mice and tumor models. In addition, IP administration can be a practical alternative in the late phase, although the initial uptake is lower than those in the IV and RO groups.