Age- and site-associated biomechanical weakening of human articular cartilage of the femoral condyle
Age- and site-associated biomechanical weakening of human articular cartilage of the femoral condyle
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DOI：
10.1016/j.joca.2007.03.005
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发表时间：
2007-09-01
影响因子：
7
通讯作者：
Sah, R. L.
中科院分区：
文献类型：
--
作者：
Temple, M. M.;Bae, W. C.;Sah, R. L.
目的：确定与宏观正常成年人膝关节软骨与年龄相关的拉伸减弱相关的生化和结构事件的时间顺序，并假设其是其基础。方法：来自年轻（21-39 岁）、中年（40-59 岁）和老年（60 岁）年龄供体的外侧和内侧股骨髁（LFC 和 MFC）的宏观正常人类关节软骨分析了拉伸性能、表面磨损以及细胞和基质组成。结果：拉伸、成分和表面结构性能的变化表明与年龄相关的软骨退化的早期、中期和晚期阶段，MFC 中发生的年龄比 LFC 更早。青年组和中年组之间的差异（表明早期到中期的阶段变化）包括浅表区域的机械功能下降，拉伸完整性丧失（或较低），以及表面磨损，在使用印度墨水后，关节表面出现微弱的条纹和轻微的染色。中老年组之间的差异（表明中后期的变化）包括维持中等水平的生物力学功能、细胞结构减少和基质糖胺聚糖含量减少。组织荧光随着年龄的增长而稳定增加。 结论：许多与年龄相关的差异与早期骨关节炎中软骨退变的病理特征相同。这些发现为机械磨损、细胞死亡和酶降解在介导早期软骨退化的连续和可区分阶段的进展中的作用提供了证据。 (C) 2007 年国际骨关节炎研究协会。由爱思唯尔有限公司出版。保留所有权利。
Objective: To determine the time sequence of biochemical and structural events associated with, and hypothesized to underlie, age-associated tensile weakening of macroscopically normal adult human articular cartilage of the knee.Methods: Macroscopically normal human articular cartilage of the lateral and medial femoral condyles (LFC and MFC) from Young (21-39 yrs), Middle (40-59 yrs), and Old ( :60 yrs) age donors were analyzed for tensile properties, surface wear, and cell and matrix composition.Results: Variations in tensile, compositional, and surface structural properties were indicative of early, intermediate, and late stages of age-associated cartilage deterioration, occurring at an earlier age in the MFC than the LFC. Differences between Young and Middle age groups (indicative of early-to-intermediate stage changes) included decreased mechanical function in the superficial zone, with a loss of (or low) tensile integrity, and surface wear, with faint striations and mild staining on the articular surface after application of India ink. Differences between Middle and Old age groups (indicative of intermediate-to-late stage changes) included maintenance of moderate level biomechanical function, a decrease in cellularity, and a decrease in matrix glycosaminoglycan content. Tissue fluorescence increased steadily with age.Conclusions: Many of these age-associated differences are identical to those regarded as pathological features of cartilage degeneration in early osteoarthritis. These findings provide evidence for the roles of mechanical wear, cell death, and enzymatic degradation in mediating the progression through successive and distinguishable stages of early cartilage deterioration. (C) 2007 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.