Molecular detection of tick-borne pathogens in cattle from Southwestern Ethiopia.
Molecular detection of tick-borne pathogens in cattle from Southwestern Ethiopia.
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DOI：
10.1371/journal.pone.0188248
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Nijhof AM
中科院分区：
文献类型：
--
作者：
Hailemariam Z;Krücken J;Baumann M;Ahmed JS;Clausen PH;Nijhof AM
壁虱传播疾病在牲畜中造成重大损失，并影响世界各地资源匮乏的农业社区的生计。在埃塞俄比亚，使用灵敏的分子检测方法对牛体内的硬虱传播病原体(TBP)的流行病学进行的详细研究很少。本研究的目的是确定具有兽医意义的牛传染性支气管炎在当地牛种群中的流行情况和种类组成。2013年6月至8月，在埃塞俄比亚西南部伊卢巴博尔地区的牛种群中进行了一项全面的横断面流行病学研究。为此，采集了392头牛的血液样本。采用聚合酶链式反应(PCR)和反向斑点杂交(RLB)相结合的方法检测样本中的TBP。392份血样的聚合酶链式反应/聚合酶链式反应结果显示，总感染率高达96.9%，其中包括变形泰勒氏菌(66.1%)、东方泰勒氏菌(51.8%)、无浆菌。Omatjenne(25.5%)、边缘无浆虫(14.5%)、巴贝斯虫(14.0%)和泰勒氏菌(13.0%)，以及少量的反转录埃里希菌(0.5%)和微小埃利希菌(0.26%)。此外，在牛血样本中还检测到三种新的无浆体基因。系统发育分析显示，它们很可能代表了三个，但至少是两个新物种。初步定名为无浆体属的三个新无浆体种的流行情况。Hadesa，无浆菌。Saso和无浆菌Saso和无浆菌。Dedessa分别为12.5%、14.3%和5.6%。总体而言，共有227头牛(57.9%)被发现同时感染两种或两种以上的TBP，并观察到86种不同的物种组合。调查结果表明，在埃塞俄比亚，牛感染结核病的负担非常高。混合感染的高频率表明，临床表现可能很复杂。需要进一步的研究来确定本研究中鉴定的三个新的无浆体物种的致病性、宿主细胞类型和媒介。
Tick-borne diseases (TBDs) cause significant losses among livestock and impact the livelihoods of resource-poor farming communities worldwide. In Ethiopia, detailed studies on the epidemiology of tick-borne pathogens (TBPs) in cattle using sensitive molecular detection methods are scarce. The objective of this study was to determine the prevalence and species composition of bovine TBPs of veterinary significance in local cattle populations. A comprehensive cross-sectional epidemiological study was conducted in cattle populations of Illubabor zone in Southwestern Ethiopia from June to August 2013. For this purpose, blood samples were collected from 392 cattle. A combination of polymerase chain reaction (PCR) and a Reverse Line Blot (RLB) hybridization assay was employed for the detection of TBPs in these samples. The PCR/RLB results of the 392 blood samples indicated a high overall prevalence of 96.9% for TBPs, including Theileria mutans (66.1%), Theileria orientalis (51.8%), Anaplasma sp. Omatjenne (25.5%), Anaplasma marginale (14.5%), Babesia bigemina (14.0%) and Theileria velifera (13.0%) and minor occurrences of Ehrlichia ruminantium (0.5%) and Ehrlichia minasensis (0.26%). Moreover, three novel Anaplasma genotypes were detected in bovine blood samples. A phylogenetic analysis revealed that they most likely represent three, but at least two, new species. The prevalence of the three novel Anaplasma species, preliminary designated as Anaplasma sp. Hadesa, Anaplasma sp. Saso and Anaplasma sp. Dedessa, was 12.5%, 14.3% and 5.6%, respectively. Overall, a total of 227 cattle (57.9%) were found to be co-infected with two or more TBPs simultaneously and 86 different species combinations were observed. The findings show a very high burden of infection of cattle with TBPs in Ethiopia. The high frequency of co-infections suggests that clinical manifestations might be complex. Further research is required to determine the pathogenicity, host cell types and vector of the three novel Anaplasma species identified in this study.
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作者：
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