Quantitative divergence of the bacterial root microbiota in Arabidopsis thaliana relatives
Quantitative divergence of the bacterial root microbiota in Arabidopsis thaliana relatives
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DOI：
10.1073/pnas.1321597111
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发表时间：
2014-01-14
影响因子：
11.1
通讯作者：
Schulze-Lefert, Paul
中科院分区：
文献类型：
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作者：
Schlaeppi, Klaus;Dombrowski, Nina;Schulze-Lefert, Paul
植物在与土壤的接触区寄生有一种独特的根相关细菌微生物群，据信其在植物营养和健康中起作用。我们调查了根微生物群的多样性内的系统发育框架的主机：三个拟南芥生态型沿着与其姐妹种拟南芥halleri和拟南芥lyrata，以及碎米荠，从前者类似于35 Mya分歧。我们在受控的环境条件下调查了他们的微生物群，并对A. thaliana和C.在两个自然栖息地的多毛类。根和相应的土壤样品的深16S rRNA基因分析确定了总共237个可量化的细菌核糖体型，其中平均73个社区成员富集在根中。这种根微生物群的组成更多地取决于与环境的相互作用，而不是与宿主物种的相互作用。宿主物种间的微生物群多样性主要是定量的，并且在三种拟南芥物种之间比三种拟南芥物种之间更大。拟南芥生态型在个体群落成员、分类群和整个根群落的水平上鉴定了宿主物种特异性微生物群。大多数这些签名被观察到在遗传学上遥远的C。多毛。然而，分支的主机系统发育顺序是不一致的种间根微生物多样性，表明主机系统发育距离不能单独解释根微生物多样性。我们的工作揭示了在35我的主机分歧很大程度上保守和分类狭窄的根微生物群，其中包括稳定的社区成员属于放线菌目，伯克霍尔德菌目，黄杆菌目。
Plants host at the contact zone with soil a distinctive root-associated bacterial microbiota believed to function in plant nutrition and health. We investigated the diversity of the root microbiota within a phylogenetic framework of hosts: three Arabidopsis thaliana ecotypes along with its sister species Arabidopsis halleri and Arabidopsis lyrata, as well as Cardamine hirsuta, which diverged from the former similar to 35 Mya. We surveyed their microbiota under controlled environmental conditions and of A. thaliana and C. hirsuta in two natural habitats. Deep 16S rRNA gene profiling of root and corresponding soil samples identified a total of 237 quantifiable bacterial ribotypes, of which an average of 73 community members were enriched in roots. The composition of this root microbiota depends more on interactions with the environment than with host species. Interhost species microbiota diversity is largely quantitative and is greater between the three Arabidopsis species than the three A. thaliana ecotypes. Host species-specific microbiota were identified at the levels of individual community members, taxonomic groups, and whole root communities. Most of these signatures were observed in the phylogenetically distant C. hirsuta. However, the branching order of host phylogeny is incongruent with interspecies root microbiota diversity, indicating that host phylogenetic distance alone cannot explain root microbiota diversification. Our work reveals within 35 My of host divergence a largely conserved and taxonomically narrow root microbiota, which comprises stable community members belonging to the Actinomycetales, Burkholderiales, and Flavobacteriales.