Analysis of cell-based RNAi screens.
Analysis of cell-based RNAi screens.
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DOI：
10.1186/gb-2006-7-7-r66
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发表时间：
2006
期刊：
影响因子：
12.3
通讯作者：
Huber W
中科院分区：
文献类型：
--
作者：
Boutros M;Brás LP;Huber W
cellHTS是一种分析和记录RNAi筛选的新方法。RNA干扰（RNAi）筛选是一种强有力的生物学途径的功能表征技术。RNAi筛选的解释需要计算和统计分析技术。我们描述了一种方法，它集成了所有步骤，从原始数据生成一个评分表型列表。它在开源的Bioconductor/R包cellHTS（）中实现。该方法可用于分析和记录单个RNAi筛选。此外，它是多个实验整合的先决条件。
cellHTS is a new method for the analysis and documentation of RNAi screens. RNA interference (RNAi) screening is a powerful technology for functional characterization of biological pathways. Interpretation of RNAi screens requires computational and statistical analysis techniques. We describe a method that integrates all steps to generate a scored phenotype list from raw data. It is implemented in an open-source Bioconductor/R package, cellHTS (). The method is useful for the analysis and documentation of individual RNAi screens. Moreover, it is a prerequisite for the integration of multiple experiments.