Regulations of m6A methylation on tomato fruit chilling injury
Regulations of m6A methylation on tomato fruit chilling injury
复制标题
m6A甲基化对番茄果实冷害的调控
DOI：
10.1016/j.hpj.2021.05.005
复制
发表时间：
2021-08-25
影响因子：
5.7
通讯作者：
Zuo, Jinhua
中科院分区：
文献类型：
--
作者：
Bai, Chunmei;Fang, Minghuan;Zuo, Jinhua
番茄果实在冷藏过程中对冷害较为敏感。番茄果实冷害的发生与多种因素有关。植物激素在其中起着重要的调控作用，但植物激素途径中转录本N-6-甲基腺苷（m（6）A）甲基化与冷害的关系尚未见报道。为了阐明m（6）A甲基化对番茄果实冷害的复杂调控机制，采用Nanopore直接测序技术对番茄果实进行了研究。大量的酶和转录因子参与了果实冷害的调控过程，这些酶和转录因子与植物激素有关，如1-氨基环丙烷1-羧酸合成酶（ACS）、天冬氨酸转氨酶（AST）、生长素反应因子（ARF 2）、乙烯反应因子（ERF 2）、赤霉素20-氧化酶-3（GA 20 ox）和茉莉酸（JA）等。通过对与低温伤害相关的差异表达转录本和m6 A甲基化差异表达转录本的联合分析，共鉴定出41个与低温伤害相关的差异表达转录本，包括1-氨基环丙烷-1-羧酸氧化酶（ACO）和果胶酯酶（PE）的表达下调以及热激同源70 kD蛋白2（cpHSC 70）的表达下调，HSP 70结合蛋白（HspBP）和水杨酸结合蛋白2（SABP 2）表达上调。研究结果为番茄果实冷害调控机制和采后冷藏提供了理论依据。
Tomato fruit are sensitive to chilling injury (CI) during cold storage. Several factors have been discovered to be involved in chilling injury of tomato fruit. Plant hormones play an important regulatory role, however, the relationship between chilling injury and N-6-methyladenosine (m(6)A) methylation of transcripts in plant hormone pathways has not been reported yet. In order to clarify the complex regulatory mechanism of m(6)A methylation on chilling injury in tomato fruit, Nanopore direct RNA sequencing was employed. A large number of enzymes and transcription factors were found to be involved in the regulation process of fruit chilling injury, which were associated with plant hormone, such as 1-aminocyclopropane 1-carboxylate synthase (ACS ), aspartate aminotransferase (AST ), auxin response factor (ARF2), ethylene response factor 2 (ERF2), gibberellin 20-oxidase-3 (GA20ox) and jasmonic acid (JA ). By conjoint analysis of the differential expression transcripts related to chilling injury and m6A methylation differential expression transcripts 41 differential expression transcripts were identified involved in chilling injury including 1-aminocyclopropane-1-carboxylate oxidase (ACO) and pectinesterase (PE ) were down-regulated and heat shock cognate 70 kD protein 2 (cpHSC70), HSP70-binding protein (HspBP) and salicylic acid-binding protein 2 (SABP2) were up-regulated. Our results will provide a deeper understanding for chilling injury regulatory mechanism and post-harvest cold storage of tomato fruit.