Bacterial cellulose with microencapsulated antifungal essential oils: A novel double barrier release system
Bacterial cellulose with microencapsulated antifungal essential oils: A novel double barrier release system
复制标题
含微胶囊抗真菌精油的细菌纤维素： 新型双屏障释放系统
DOI：
10.1016/j.mtla.2020.100585
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发表时间：
2020-03-01
期刊：
影响因子：
3.4
通讯作者：
Khandelwal, Mudrika
中科院分区：
文献类型：
--
作者：
Adepu, Shivakalyani;Khandelwal, Mudrika
双屏障释放系统是为具有巨大抗真菌活性的草本精油设计的，甚至对耐药真菌也是如此。然而，由于它们对pH、温度和光的敏感性，它们的利用受到了限制。将三种精油(百里香酚、丁香酚、香芹酚)微胶囊化在聚乳酸壳中，其包封率高达80%，以充分发挥它们的协同抗真菌活性。这些微胶囊进一步被结合到细菌纤维素中--一种天然产生的纳米纤维水凝胶。这一独特的系统为油的释放提供了双重屏障--聚合物外壳和纳米纤维基质。对模型真菌--白色念珠菌(常见口腔、阴道感染的病原菌)进行抗真菌试验。体外经阴道药物释放研究比较了胶体微囊和复合微囊的释药情况，后者表现为控释。通过数学模拟来理解骨架溶胀、油的扩散和微胶囊的侵蚀所控制的释放。时间-杀灭曲线表明，与胶体微胶囊相比，该复合材料能够在油用量减半的情况下达到杀菌终点，且远高于商品抗真菌药物氟康唑。
A double barrier release system is designed for herbal essential oils which possess tremendous anti-fungal activity, even against drug-resistant fungi. However, their utilization is limited owing to their sensitivity towards pH, temperature and light. Three essential oils (thymol, eugenol, carvacrol) are microencapsulated in polylactic acid shell with a high encapsulation efficiency of 80% in order to leverage their synergistic anti-fungal activity. These microcapsules are further incorporated in bacterial cellulose - a naturally produced nanofibrous hydrogel. This unique system offers double barrier to the release of oils - the polymeric shell and the nanofibrous matrix. The anti-fungal testing was carried out on model fungus- Candida albicans (cause of common oral, vaginal infections). In vitro transvaginal drug release study was performed to compare release from microcapsules as colloids and the composites, where the later exhibited a controlled release. Mathematical modelling was performed to understand the release, which is governed by matrix swelling, diffusion of oils and erosion of microcapsules. The time-kill curves show that the composites were able to achieve fungicidal endpoint with half the oil dosage as compared to the colloidal microcapsules and were far more effective than commercial antifungal fluconazole.