Age-related changes in kallikreins-kinins system in rat corpus cavernosum.
Age-related changes in kallikreins-kinins system in rat corpus cavernosum.
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DOI：
10.1111/j.1365-2605.2010.01052.x
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发表时间：
2011-02
影响因子：
--
通讯作者：
T. Wang;Z. Wan;J. Liu;M. Chen;R. B. Chen;W. Yang;Z. Ye
中科院分区：
文献类型：
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作者：
T. Wang;Z. Wan;J. Liu;M. Chen;R. B. Chen;W. Yang;Z. Ye
一氧化氮合酶、雄激素和生长因子等多种因素对阴茎海绵体平滑肌张力的调节具有年龄相关性。激肽释放酶-激肽系统（KKS）的活性代谢产物缓激肽、Lys-BK和Met-Lys-BK在体外也能显著舒张CC平滑肌。我们的目的是评估大鼠CC中KKS和年龄之间的特定关联。分别在出生后2、8、12、20、30、40和60周（PW）分离大鼠CC和胸主动脉。实时荧光定量PCR检测CC和胸主动脉组织激肽释放酶I（KLKI）和激肽B2受体（B2 R）mRNA的表达。免疫荧光原位杂交和Western blot检测KLKI和B2 R蛋白表达。实时荧光定量PCR、原位免疫荧光和Western blot结果均显示，CC和胸主动脉中KLKI表达的增龄性变化相似。PW 2 ~ PW 30随年龄增长而显著增加，PW 30时达高峰，随后逐渐下降。然而，PW 30、PW 40和PW 60之间的差异无统计学意义。B2 R的表达随着年龄的增长而逐渐增加，在成年期达高峰，在PW 20、PW 30、PW 40之间差异不显著，PW 60时表达显著下降。CC和年龄匹配的主动脉中B2 R的表达变化相似，但在PW 60时显著低于主动脉。KLKI和B2 R的表达在大鼠CC中以年龄依赖性模式变化，并且在衰老过程中有下降的趋势，这与报道的老年男性勃起能力的趋势相同，并在一定程度上表明为什么衰老是艾德的独立预测因子。
Many factors, such as nitric oxide synthase, androgen and growth factors, can regulate the tone of corpus cavernosum (CC) smooth muscle with an age-related tendency. It has been shown that the active metabolites of kallikreins-kinins system (KKS), including bradykinin, Lys-BK and Met-Lys-BK, can also relax the CC smooth muscle significantly in vitro. Our aim was to evaluate the specific association between KKS and age in rat CC. CC and thoracic aorta were isolated from rats at postnatal weeks (PW) of 2, 8, 12, 20, 30, 40 and 60, respectively. Tissue kallikrein-I (KLKI) and kinin B2 receptor (B2R) mRNA in CC and thoracic aorta were detected by real-time polymerase chain reaction (PCR). Protein expression of KLKI and B2R were determined with immunofluorescence in situ and Western blot. Real-time PCR, immunofluorescence in situ and Western blot all demonstrated that the age-related changes in expression of KLKI were similar between the CC and thoracic aorta. It significantly increased with age from PW2 to PW30, reached the peak at PW30 and then declined gradually. However, there was no statistically significant difference among PW30, PW40 and PW60. Similarly, the expression of B2R increased gradually with age reached and remained at the peak during adult stages and no significant differences were found among PW20, PW30 and PW40; then, it decreased significantly at PW60. The changes in the expression of B2R in CC and age-matched aorta were similar except that it was significantly less than that in the aorta at PW60. The expression of KLKI and B2R changed in an age-dependent pattern in rat CC and have a tendency to decline during ageing, which is of the same tendency as reported for erection capacity in ageing males and suggests why ageing is an independent predictor of ED, to some extent.