Determination of the pH gradient across the stratum corneum.
Determination of the pH gradient across the stratum corneum.
复制标题
测定角质层的 pH 梯度。
DOI：
10.1038/jidsymp.1998.23
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Guy,RH
中科院分区：
文献类型：
--
作者：
Turner,NG;Cullander,C;Guy,RH
这项工作的目的是使用比率激光扫描共焦显微镜成像和平面电极直接 pH 测量来确定无毛小鼠角质层 (SC) 的 pH 梯度分布。双发射比率成像使用荧光团羧基半萘霍达氟 1，其发射光谱显示出 pH 依赖性变化。然而，开发的测定方法并不成功，因为 (i) 半萘霍达氟的 pKaof (约 7.5) 使其对哺乳动物 SC 的正常 pH 梯度 (4-7.4) 不够敏感，以及 (ii) 皮肤出乎意料的高缓冲能力妨碍了对系统进行有意义的校准。在第二种方法中，当无毛小鼠 SC 被逐渐剥离胶带时，用平面电极记录 pH 测量值。在表层 SC 中，pH 值保持相对恒定（约 5.9）；进一步去除屏障导致pH值稳定增加至约7。总之，显然需要改进的方法来精确表征整个 SC 的 pH 曲线。Journal of Investigative Dermatology Symposium Proceedings 3:110–113, 1998
The objective of this work was to determine the pH gradient profile across hairless mouse stratum corneum (SC) using ratiometric laser scanning confocal microscopy imaging and direct pH measurement with a flat surface electrode. Dual-emission ratiometric imaging used the fluorophore, carboxy seminaphthorhodafluor1, which displays a pH-dependent shift in its emission spectrum. The assay developed was unsuccessful, however, because (i) the pKaof seminaphthorhodafluor (≈7.5) makes it insufficiently sensitive to the normal pH gradient (4–7.4) across mammalian SC, and (ii) the unexpectedly high buffering capacity of the skin precluded a meaningful calibration of the system. In the second method, pH measurements with a flat surface electrode were recorded as the hairless mouse SC was progressively tape stripped. In the superficial SC, the pH remained relatively constant (≈5.9); further removal of the barrier resulted in a steady increase in pH to ≈7. In conclusion, improved methodology is clearly required to characterize precisely the pH profile across the SC.Journal of Investigative Dermatology Symposium Proceedings 3:110–113, 1998