A novel cofactor for p300 that regulates the p53 response
A novel cofactor for p300 that regulates the p53 response
复制标题
DOI：
10.1016/s1097-2765(00)80338-x
复制
发表时间：
1999-09-01
期刊：
影响因子：
16
通讯作者：
La Thangue, NB
中科院分区：
文献类型：
--
作者：
Shikama, N;Lee, CW;La Thangue, NB
p53 作为转录因子的能力有助于促进对细胞应激的反应，而 p300/CBP 蛋白作为多种转录因子的共激活剂，参与调节 p53 活性。我们报告了一种新的 p300 辅助因子，它通过增强 p53 依赖性转录和细胞凋亡来促进 p53 反应。 JMY 和 p300 在生理条件下结合，并且在细胞应激反应期间，p300/JMY 复合物被招募到激活的 p53。 bar 基因被 JMY 有效激活，通过选择性剪接产生的蛋白质异构体改变了 p53 反应的功能结果。结果提供了令人信服的证据，证明 p300/JMY 共激活剂复合物在促进 p53 反应中发挥着核心作用。
The ability of p53 to function as a transcription factor is instrumental in facilitating the response to cellular stress, and p300/CBP proteins, which act as coactivators for diverse transcription factors, participate in regulating p53 activity. We report a novel cofactor for p300 that facilitates the p53 response by augmenting p53-dependent transcription and apoptosis. JMY and p300 associate in physiological conditions, and, during the cellular stress response, the p300/JMY complex is recruited to activated p53. The bar gene is efficiently activated by JMY, and protein isoforms that arise through alternative splicing alter the functional outcome of the p53 response. The results provide compelling evidence that the p300/JMY coactivator complex plays a central role in facilitating the p53 response.