Chemotactic response of rat mammary adenocarcinoma cell clones to tumor-derived cytokines.
Chemotactic response of rat mammary adenocarcinoma cell clones to tumor-derived cytokines.
复制标题
大鼠乳腺癌细胞克隆对肿瘤源性细胞因子的趋化反应。
DOI：
10.1016/0006-291x(87)90746-7
复制
发表时间：
1987
影响因子：
3.1
通讯作者：
Dabbous,MK
中科院分区：
文献类型：
--
作者：
Atnip,KD;Carter,LM;Nicolson,GL;Dabbous,MK
从 MTLn3 细胞条件化的无血清培养基或 MTLn3 细胞匀浆（大鼠 13762NF 乳腺癌的高度转移变体）中分离出表观分子量为 53,000 道尔顿的细胞因子。使用改良的博伊登室在体外测试了 MTLn3 和低转移变体 MTLn2 细胞对此细胞因子的趋化反应。 MTLn3 细胞的趋化运动和趋化运动均受到 MTLn3 衍生细胞因子的刺激。此外，MTLn3衍生的细胞因子刺激MTLn2肿瘤细胞相对较小但显着的趋化迁移，而这些细胞对MTLn2细胞条件化的培养基没有反应。 MTLn3 细胞本身对 I 型胶原或 MTLn2 细胞条件化的培养基没有趋化反应。这些结果表明，趋化反应可能是入侵肿瘤细胞转移潜能的函数。因此，增强肿瘤细胞运动性的肿瘤细胞因子的产生可能代表高转移潜能和低转移潜能的13762NF肿瘤细胞亚群之间的表型差异。
A cytokine with an apparent molecular weight of 53,000 daltons was isolated from serum-free medium conditioned by MTLn3 cells or from homogenates of MTLn3 cells, a highly metastatic variant of the rat 13762NF mammary adenocarcinoma. The chemotactic responses of MTLn3 and the low metastatic variant MTLn2 cells to this cytokine were tested in vitro using modified Boyden chambers. Both the chemotactic and chemokinetic movements of MTLn3 cells were stimulated by the MTLn3-derived cytokine. In addition, the MTLn3-derived cytokine stimulated a relatively small, but significant chemotactic migration of MTLn2 tumor cells, while these cells did not respond to medium conditioned by MTLn2 cells. MTLn3 cells themselves did not respond chemotactically to type I collagen or medium conditioned by MTLn2 cells. These results suggest that the chemotactic response may be a function of metastatic potential of the invading tumor cells. The production of tumor cytokines that enhance tumor cell motility may thus represent a phenotypic difference between 13762NF tumor cell subpopulations of high and low metastatic potential.