THE DEPENDENCE OF NERVE REGENERATION THROUGH MUSCLE GRAFTS IN THE RAT ON THE AVAILABILITY AND ORIENTATION OF BASEMENT-MEMBRANE
THE DEPENDENCE OF NERVE REGENERATION THROUGH MUSCLE GRAFTS IN THE RAT ON THE AVAILABILITY AND ORIENTATION OF BASEMENT-MEMBRANE
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DOI：
10.1007/bf01611732
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发表时间：
1986-08-01
期刊：
影响因子：
--
通讯作者：
HUANG, CLH
中科院分区：
文献类型：
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作者：
GLASBY, MA;GSCHMEISSNER, SG;HUANG, CLH
通过移植的基底膜基质，制备冷冻自体肌肉，然后在蒸馏水中解冻，神经再生进行了研究，在Sprague-Dawley大鼠。移植物的基底膜管与切断的坐骨神经的近端和远端同轴，在移植后51天观察到远端神经恢复的电生理证据。这与移植物和远端神经内发育良好的有髓神经纤维的组织学发现相关。然而，尽管3个月后移植物中的轴突数量达到正常，但这些肌肉移植物中的再生神经需要6个月至1年才能恢复正常的轴突直径和髓鞘形成。移植物的基底膜管与神经纤维成直角，以及未处理的肌肉移植物同轴排列，延迟了恢复。它的结论是，成功的远端残端和功能恢复可以遵循神经再生，通过治疗肌肉自体移植。再生的速率取决于空的基底膜管的可用性。如果这些是不可用的或不适当的定向，再生仍然可以发生，但显着延迟。
Nerve regeneration through grafts of basement membrane matrix, prepared by freezing of autogenous muscle followed by thawing in distilled water, was investigated in Sprague-Dawley rats. Electrophysiological evidence of recovery in distal nerve was observed at 51 days after implantation of treated grafts whose basement membrane tubes were coaxial with the proximal and distal ends of the transected sciatic nerve. This correlated with histological findings of well-developed myelinated nerve fibers within both grafts and distal nerve. However, whereas normal axon numbers were achieved in the grafts by 3 months, the regenerating nerve in these muscle grafts took 6 months to 1 year to recover normal axon diameter and myelination. Recovery was delayed through grafts whose basement membrane tubes were at right angles to the nerve fibers and through grafts of untreated muscle coaxially aligned. It is concluded that successful repopulation of the distal stump and functional recovery can follow nerve regeneration through treated muscle autografts. The rate of regeneration is dependent on the availability of empty basement membrane tubes. If these are unavailable or inappropriately orientated, regeneration can still occur but is significantly delayed.