Proteomics Profiling of Pancreatic Cancer and Pancreatitis for Biomarkers Discovery.
Proteomics Profiling of Pancreatic Cancer and Pancreatitis for Biomarkers Discovery.
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DOI：
10.4172/2157-7013.1000287
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发表时间：
2018-01-01
期刊：
影响因子：
--
通讯作者：
Mohammed, S I
中科院分区：
文献类型：
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作者：
Sanh, N;Fadul, H;Mohammed, S I
胰腺癌是最具侵袭性的恶性肿瘤之一，预计发病率会增加，特别是在非洲裔美国人中。胰腺癌被认为是一种无症状疾病，预后差，缺乏早期生物标志物进行检测。蛋白质组学已被应用于许多疾病的鉴定或发现生物标志物。长期以来，人们一直认为慢性胰腺炎可能是胰腺癌发生的危险因素。这项研究使用蛋白质组学技术鉴定了胰腺癌和胰腺炎中与正常人相比表达改变的蛋白质。从激光捕获的显微切割组织中提取蛋白质，并在2-DPAGE中分离并成像。胰腺癌和胰腺炎的蛋白质谱相似，但与正常癌旁组织的蛋白质谱不同。从凝胶中切下在肿瘤和胰腺炎中过表达但在正常组织中不过表达的代表性蛋白质，进行凝胶内消化，并通过MALDI-TOF质谱法进行分析。鉴定的蛋白质包括转铁蛋白、ER-60蛋白、前载脂蛋白、原肌球蛋白1、α 1肌动蛋白前体、ACTB蛋白和γ 2前肽、醛脱氢酶1A 1、胰脂肪酶和膜联蛋白A1。在胰腺癌中显示的几种蛋白质也在胰腺炎样品中观察到。了解这些特定蛋白质的作用及其机制作用将有助于深入了解它们与胰腺癌的关系。
Pancreatic cancer is one of the most aggressive malignancies with an increase in incidence predicted, particularly in African Americans. Pancreatic cancer is considered a silent disease with poor prognosis and a lack of early biomarkers for detection. Proteomics has been applied in many diseases for identifying or discovering biomarkers. It has long been suggested that chronic pancreatitis may be a risk factor for developing pancreatic cancer. This study identified proteins that are altered in expression in pancreatic cancer and pancreatitis compared to normal using proteomic technology. Proteins were extracted from laser captured micro-dissected tissues and separated in 2-DPAGE and imaged. The protein profiles of pancreatic cancer and pancreatitis are similar but differed with the protein profile of normal adjacent tissues. Representative proteins, overexpressed in tumor and pancreatitis but not normal tissues, were excised from gels, subjected to in-gel digestion, and analyzed by MALDI-TOF mass spectrometry. Proteins identified included transferrin, ER-60 protein, proapolipoprotein, tropomyosin 1, alpha 1 actin precursor, ACTB protein, and gamma 2 propeptide, aldehyde dehydrogenase 1A1, pancreatic lipase and annexin A1. Several proteins, which were shown in pancreatic cancer, were also observed in pancreatitis samples. Understanding the role of these specific proteins and their mechanistic action will give insights into their involvement in pancreatic cancers.