The antenna‐specific odorant‐binding protein AlinOBP13 of the alfalfa plant bug Adelphocoris lineolatus is expressed specifically in basiconic sensilla and has high binding affinity to terpenoids
The antenna‐specific odorant‐binding protein AlinOBP13 of the alfalfa plant bug Adelphocoris lineolatus is expressed specifically in basiconic sensilla and has high binding affinity to terpenoids
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DOI：
10.1111/imb.12089
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发表时间：
2014-08
影响因子：
2.6
通讯作者：
Liang Sun;Hai-Jun Xiao;S. Gu;Jing-Jiang Zhou;Y. Guo;Zifei Liu;Y.‐J. Zhang
中科院分区：
文献类型：
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作者：
Liang Sun;Hai-Jun Xiao;S. Gu;Jing-Jiang Zhou;Y. Guo;Zifei Liu;Y.‐J. Zhang
气味结合蛋白（OBPs）在昆虫的嗅觉通路中起着至关重要的作用。在本研究中，研究了天线富集的OBP AlinOBP13，因为它可能对紫花苜蓿植物臭虫Adelphocoris lineolatus的外周嗅觉感知有潜在的贡献。定量逆转录酶PCR结果显示，AlinOBP13在成虫期的转录水平高于若虫期。饥饿4 h和8 h后，雄性和雌性触角中AlinOBP13转录本水平分别显著升高。采用透射电镜和免疫细胞化学标记技术对不同类型化学感受器雌雄触角的超微结构进行了研究。结果表明，抗AlinOBP13抗血清对短基感器具有特异标记作用；该抗血清仅限于感觉器的内腔和感觉器基部以下的腔。相比之下，多孔毛感器、中长基感器和多孔感器没有标记。本研究首次报道了在半翼类动物的短基本感受器中显示特异性表达的OBP。荧光置换结合实验结果表明，重组蛋白AlinOBP13对萜类化合物的结合比对性信息素和其他化学物质的结合更具特异性。这种结合能力受到pH值的显著影响；pH值为10.0时比pH值为7.4和5.0时表现出更高的结合亲和力。此外，来自触角的剂量依赖性触角电图记录结果显示，雌性和雄性成虫都对所测试的萜类化合物有反应，这表明AlinOBP13在lineolatus化学接受中具有明显的生理相关性。本研究结果提示AlinOBP13作为萜类化合物的特异性载体，并为lineolatus对绿色挥发物的响应机制提供了新的思路。
Odorant‐binding proteins (OBPs) are crucial in the olfactory pathway of insects. In the present study, the antenna‐enriched OBP AlinOBP13 was investigated because of its potential contribution to the peripheral olfactory perception in the alfalfa plant bug Adelphocoris lineolatus. The results of quantitative reverse transcriptase‐PCR showed that the transcript level of AlinOBP13 was higher in the adult stage than in the nymph stages. The transcript levels of AlinOBP13 in the male and female antennae significantly increased after 4 and 8 h of starvation, respectively. Fine ultrastructures of different types of chemosensilla in both female and male antennae were investigated using transmission electron microscopy and immunocytochemical labelling. The results revealed that the anti‐AlinOBP13 antiserum strongly and specifically labelled short basiconic sensilla; this antiserum was restricted to the inner lumen and the cavities below the sensillum base of the sensilla. By contrast, multiporous sensilla trichodea, medium long sensilla basiconica, and aporous sensilla chaetica were not labelled. The present study is the first to report an OBP showing specific expression in the short basiconic sensilla of a member of the Hemipteran species. The results of a fluorescence displacement binding assay indicated that recombinant AlinOBP13 showed a more specific binding preference to terpenoids than to sex pheromones and other classes of chemicals. This binding ability was dramatically affected by pH; higher binding affinities were displayed at pH 10.0 than at pH 7.4 and 5.0. In addition, the results of dose‐dependent electroantennogram recordings from the antennae showed that both female and male adult bugs responded to the terpenoids tested, suggesting an apparent physiological relevance of AlinOBP13 in A. lineolatus chemoreception. The results of this study suggest that AlinOBP13 functions as a specific carrier of terpenoids and provide insights into the mechanism of A. lineolatus in response to green volatiles.