Biochemical characterization of a monoclonal antibody to the H type-2 antigen: comparison to other ABH antibodies.
Biochemical characterization of a monoclonal antibody to the H type-2 antigen: comparison to other ABH antibodies.
复制标题
H 2 型抗原单克隆抗体的生化特征：与其他 ABH 抗体的比较。
作者：
Rosenblum,BB;Judd,WJ;Carey,TE
检测了抗ABH血型抗原的单抗和多克隆抗体对溶解红细胞膜免疫印迹上具有ABH活性的糖蛋白的特异性。免疫印迹用针对H2型碳水化合物结构的单抗G10或商业上制备的针对A、B和H血型抗原的单抗和多克隆抗体进行染色。G10抗体在含有红细胞膜带3和4.5的区域特异性地染色抗原；染色与红细胞的预期H含量成比例(O>；A2>；B>；A2B>；A1>；A1B)。从缺乏H-2型结构的OH(孟买)红细胞膜中没有观察到特殊的染色。商业化制备的抗H单抗不能特异性染色红细胞膜抗原。A和AB的单克隆和多克隆抗A特异性染色条带，但不包括O、B或其他红细胞(A1>；A1B>；A2>；A2B)。多克隆抗B血清特异性染色条带来自B和AB，但不包括O、A或其他红细胞(B>；A2B>；A1B)。然而，在单抗B的试验中没有观察到特殊的染色。单抗G10和抗-A以及多克隆抗-A和抗-B血型血清将有助于进一步表征ABH抗原的分子性质。
Monoclonal and polyclonal antibodies to the ABH blood group antigens were tested for their specificity to glycoproteins with ABH activity on immunoblots of solubilized erythrocyte membranes. Immunoblots were stained with monoclonal antibody G10 to the H type-2 carbohydrate structure or with commercially prepared monoclonal and polyclonal antibodies to A, B, and H blood group antigens. G10 antibody specifically stained antigens in the regions that contain the erythrocyte membrane bands 3 and 4.5; the staining was proportional to the expected H content of the erythrocytes (O > A2> B > A2B > A1> A1B). No specific staining was observed with membranes derived from Oh(Bombay) erythrocytes which lack the H type-2 structure. A commercially prepared monoclonal anti-H did not specifically stain erythrocyte membrane antigens. Monoclonal and polyclonal anti-A specifically stained bands from A and AB but not O, B, or Oherythrocytes (A1> A1B > A2> A2B). Polyclonal anti-B serum specifically stained bands from B and AB but not O, A, or Oherythrocytes (B > A2B > A1B). However, no specific staining was observed in tests with monoclonal anti-B. Monoclonal antibodies G10 and anti-A and polyclonal anti-A and -B blood typing sera will be useful in the further characterization of the molecular nature of the ABH antigens.