MINIMIZING SAMPLE EVAPORATION IN THE ENVIRONMENTAL SCANNING ELECTRON-MICROSCOPE
MINIMIZING SAMPLE EVAPORATION IN THE ENVIRONMENTAL SCANNING ELECTRON-MICROSCOPE
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DOI：
10.1111/j.1365-2818.1994.tb03445.x
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发表时间：
1994-03-01
期刊：
影响因子：
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通讯作者：
DONALD, AM
中科院分区：
文献类型：
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作者：
CAMERON, RE;DONALD, AM
电子扫描环境扫描电子显微镜(ESEM)可以通过排除空气但允许水蒸气进入样品室来观察潮湿的样品。然而，在用于达到这种状态的泵压过程中，样品可能会蒸发和冷凝，这意味着如果不小心观察，样品可能不会处于其自然状态。本文对环境扫描电子显微镜的泵送系统进行了数学描述，并推导了蒸发和冷凝的表达式。然后使用该处理来计算最优下压序列。通过脂肪乳剂的显微照片说明了使用优化工艺的重要性。
The ElectroScan environmental scanning electron microscope (ESEM) enables wet samples to be observed by eliminating air but allowing water vapour into the sample chamber. However, evaporation from, and condensation on, the sample may occur during the pumpdown sequence used to reach this state, which means that the sample may not be in its natural state when viewed if due care is not taken. In this paper, the pumping system of the ESEM is described mathematically and expressions are derived for the evaporation and condensation. This treatment is then used to calculate the optimum pumpdown sequence. The importance of using the optimized procedure is illustrated by micrographs of fat emulsions.