Tissue inhibitor of metalloproteinase 2 and coronary artery lesions in Kawasaki disease
Tissue inhibitor of metalloproteinase 2 and coronary artery lesions in Kawasaki disease
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DOI：
10.1016/j.jpeds.2007.03.010
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发表时间：
2007-08-01
影响因子：
5.1
通讯作者：
Hara, Toshiro
中科院分区：
文献类型：
--
作者：
Furuno, Kenji;Takada, Hidetoshi;Hara, Toshiro
目的 鉴定川崎病（KD）伴冠状动脉病变（CAL）急性期外周血单个核细胞（PBMNC）中特有表达的细胞因子基因。研究设计采用DNA微阵列技术筛选了4对伴或不伴CAL的川崎病（KD）患者的PBMNC mRNA表达水平。结果通过实时聚合酶链式反应（RT-PCR）得到证实。通过遗传关联研究分析已识别基因中单核苷酸多态性对 KD 患者发生 CAL 的意义（184 名对照者、144 名患有 CAL 的 KD 患者、64 名无 CAL 的 KD 患者）。结果 mier6array 分析显示，患有 CAL 的 KD 患者的 PBMNC 中组织蛋白酶 2 抑制剂 (TIMP2) 的表达水平高于无 CAL 的 KD 患者。定量RT-PCR证实，有CAL的KD患者中表达水平显着高于无CAL的患者（P
Objective To identify cytokine genes uniquely expressed in peripheral blood mononuclear cells (PBMNCs) in the acute phase of Kawasaki disease (KD) with coronary artery lesions (CALs).Study design We screened the mRNA expression levels of PBMNCs from 4 pairs of KD patients with and without CAL using DNA microarray. The result was confirmed by real-time polymerase chain reaction (RT-PCR). The genetic association study was performed to analyze the significance of single nucleotide polymorphisms in the identified gene for the development of CAL in KD patients (184 controls, 144 KD patients with CAL, 64 KD patients without CAL).Results The mier6array analysis showed that tissue inhibitor of metafloproteinases 2 (TIMP2) was expressed at higher levels in PBMNCs of KD patients with CAL than in KD patients without CAL. Quantitative RT-PCR confirmed that the expression levels were significantly higher in the KD patients with CAL than in those without CAL (P