Reverse genetic analysis of the transcription regulatory sequence of the coronavirus transmissible gastroenteritis virus
Reverse genetic analysis of the transcription regulatory sequence of the coronavirus transmissible gastroenteritis virus
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DOI：
10.1128/jvi.78.11.6061-6066.2004
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发表时间：
2004-06-01
影响因子：
5.4
通讯作者：
Baric, RS
中科院分区：
文献类型：
--
作者：
Curtis, KM;Yount, B;Baric, RS
冠状病毒不连续转录在前导 RNA 和体 RNA 的连接中使用高度保守的序列 (CS)。使用传染性胃肠炎病毒的全长感染性构建体，本研究表明亚基因组转录受到上游侧翼序列的严重影响，并支持转录减弱机制，该机制部分受到主要由上游侧翼序列组成的更大结构域的调节，该结构域选择适当位置的CS元件来合成亚基因组RNA。
Coronavirus discontinuous transcription uses a highly conserved sequence (CS) in the joining of leader and body RNAs. Using a full-length infectious construct of transmissable gastroenteritis virus, the present study demonstrates that subgenomic transcription is heavily influenced by upstream flanking sequences and supports a mechanism of transcription attenuation that is regulated in part by a larger domain composed of primarily upstream flanking sequences which select appropriately positioned CS elements for synthesis of subgenomic RNAs.