Microsatellites confirm the authenticity of inter-varietal chromosome substitution lines of wheat (Triticum aestivum L.)
Microsatellites confirm the authenticity of inter-varietal chromosome substitution lines of wheat (Triticum aestivum L.)
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DOI：
10.1007/s001220051455
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发表时间：
2000-07
影响因子：
5.4
通讯作者：
E. Pestsova;E. Salina;A. Börner;V. Korzun;O. Maystrenko;M. Röder
中科院分区：
文献类型：
--
作者：
E. Pestsova;E. Salina;A. Börner;V. Korzun;O. Maystrenko;M. Röder
利用95个小麦微卫星标记（WMS）验证了以Saratovskaya 29为受体品种开发的Saratovskaya 29/Yanetzkis Probat品种间小麦染色体代换系的真实性。除4DS、6DS和7 DS外，其余染色体臂均存在多态性标记。每个染色体代换系用2-8个微卫星标记进行检测。结果表明，大多数线条是正确的。在21个供试品系中，有17个表现出了供体品种的预期微卫星模式。两个完整的染色体，1B和7A，和两个染色体臂，3AL和6DS，没有取代Yanetzkis Probat在各自的线。位于相应代换系染色体臂4AL、3BS和5 BL远侧区的3个微卫星标记未显示受体品种的预期微卫星模式。讨论了不正确的代换系开发和不正确的远端微卫星标记的出现的可能原因。这些数据证实了微卫星标记为检测小麦遗传资源的真实性提供了理想的工具。
Ninety-five wheat microsatellite markers (WMS) were used to verify the authenticity of the set of Saratovskaya 29/Yanetzkis Probat inter-varietal wheat chromosome substitution lines developed using Saratovskaya 29 as the recipient variety. Polymorphic markers were available for all chromosome arms except 4DS, 6DS and 7DS. Each chromosome substitution line was tested by 2–8 microsatellite markers. The results demonstrate that most of the lines are correct. Out of 21 lines tested 17 showed the expected microsatellite pattern of the donor variety. Two entire chromosomes, 1B and 7A, and two chromosome arms, 3AL and 6DS, were not substituted with Yanetzkis Probat in their respective lines. Three microsatellite markers located in the distal regions of chromosome arms 4AL, 3BS and 5BL in the corresponding substitution lines did not reveal the expected microsatellite pattern of the recipient variety. The possible causes of the incorrect substitution line development and the appearance of incorrect distal microsatellite markers are discussed. The data confirm the idea that microsatellite markers provide ideal tools for testing the authenticity of genetic stocks of wheat.