Acoustic matrix microseeding: improving protein crystal growth with minimal chemical bias
Acoustic matrix microseeding: improving protein crystal growth with minimal chemical bias
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DOI：
10.1107/s0907444910005512
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发表时间：
2010-05-01
影响因子：
2.2
通讯作者：
Harris, Seth F.
中科院分区：
文献类型：
--
作者：
Villasenor, Armando G.;Wong, April;Harris, Seth F.
介绍了一种晶体播种技术，它使用声波将纳升体积的种子悬浮液输送到蛋白质液滴中。输送体积的减少可以在基质播种实验中促进晶体生长，而无需担心种子携带缓冲液悬浮液中的化学成分造成的偏差。利用这项技术，研究发现，虽然在某些情况下，仅有缓冲成分而没有种子可以略微促进晶体的生长，但晶体种子在增加稀疏基质条件下产生蛋白质晶体的数量方面要有效得多。
A crystal seeding technique is introduced that uses acoustic waves to deliver nanolitre volumes of seed suspension into protein drops. The reduction in delivery volume enables enhanced crystal growth in matrix-seeding experiments without concern for bias from chemical components in the seed-carrying buffer suspension. Using this technique, it was found that while buffer components alone without seed can marginally promote crystal growth in some cases, crystal seeding is far more effective in boosting the number of sparse-matrix conditions that yield protein crystals.