Eukaryotic major facilitator superfamily transporter modeling based on the prokaryotic GlpT crystal structure (Review)
Eukaryotic major facilitator superfamily transporter modeling based on the prokaryotic GlpT crystal structure (Review)
复制标题
基于原核 GlpT 晶体结构的真核主要易化子超家族转运蛋白建模（综述）
DOI：
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发表时间：
2007
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通讯作者：
M. Joanne Lemieux
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文献类型：
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作者：
M. Joanne Lemieux
转运蛋白的主要易化剂超家族（MFS）代表次级主动转运蛋白的最大家族，并且具有多种底物。与四个MFS转运蛋白的结构信息，我们可以看到一个强大的结构共性建议，如预测，一个共同的架构MFS转运蛋白。MFS转运蛋白的晶体结构测定速度缓慢，使得原核和真核转运蛋白的建模更加诱人。本文综述了基于原核GlpT晶体结构和基于LacY晶体结构的真核转运蛋白Glut1、G6PT、OCT 1、OCT 2和Pho84的模型。用于生成不同的模型的技术进行了比较。此外，这些模型的有效性和使用原核晶体结构的真核蛋白质模型的策略进行了讨论。作为比较，E. coliGlpT模型是基于E. coli LacY结构，并与GlpT的晶体结构进行比较，表明实验证据对于膜蛋白的精确建模是必不可少的。
The major facilitator superfamily (MFS) of transporters represents the largest family of secondary active transporters and has a diverse range of substrates. With structural information for four MFS transporters, we can see a strong structural commonality suggesting, as predicted, a common architecture for MFS transporters. The rate for crystal structure determination of MFS transporters is slow, making modeling of both prokaryotic and eukaryotic transporters more enticing. In this review, models of eukaryotic transporters Glut1, G6PT, OCT1, OCT2 and Pho84, based on the crystal structures of the prokaryotic GlpT, based on the crystal structure of LacY are discussed. The techniques used to generate the different models are compared. In addition, the validity of these models and the strategy of using prokaryotic crystal structures to model eukaryotic proteins are discussed. For comparison, E. coli GlpT was modeled based on the E. coli LacY structure and compared to the crystal structure of GlpT demonstrating that experimental evidence is essential for accurate modeling of membrane proteins.