GABA-ergic interneurons involved in transcallosal inhibition of the visual cortices in vivo in mice
GABA-ergic interneurons involved in transcallosal inhibition of the visual cortices in vivo in mice
复制标题
GABA能中间神经元参与小鼠体内视觉皮层的经胼胝体抑制
DOI：
10.1016/j.physbeh.2015.08.026
复制
发表时间：
2015-11-01
影响因子：
2.9
通讯作者：
Pei, Zhong
中科院分区：
文献类型：
--
作者：
He, Xiao-fei;Lan, Yue;Pei, Zhong
In the current study we investigated the role of the corpus callosum, particularly the gamma-aminobutyric acid-ergic (GABAergic) projection neurons involved in interhemispheric inhibition (IHI). In order to explore IHI in primary visual cortices, we adopted a protocol whereby we performed a direct current lesion of the unilateral primary visual cortex with or without posterior callosotomy, and used two-photon Ca2+ in vivo imaging on the opposite unaffected region to detect neural activities in mice. Following this procedure, the numbers of vesicular GABAergic transporters (VGATs) and GABAergic interneurons in the unaffected primary cortex were determined using immunofluorescence staining. Results indicated that following unilateral visual cortical lesioning without callosotomy, the neuronal Ca2+ activities in the opposite side were significantly increased. However, the neuronal activities of the unaffected visual cortex in animals with unilateral cortical lesion with callosotomy were not significantly different. Additionally, there was no significant difference in the numbers of GABAergic interneurons in the unaffected region between each group, while the number of VGATs in the unaffected region was significantly decreased following unilateral visual cortical lesion without callosotomy, which was unchanged once with callosotomy. Finally, callosotomy alone without cortical lesioning produced no change in neuronal activities, the number of GABAergic interneurons or VGATs. Our results demonstrate that IHI between the homologous primary visual cortices occurs via the corpus callosum, and further indicate the important involvement of long-range GABAergic interneurons in transcallosal inhibition. (C) 2015 Elsevier Inc. All rights reserved.
