SUBPOPULATIONS OF MATURE MURINE THYMOCYTES - PROPERTIES OF CD4-CD8+ AND CD4+CD8- THYMOCYTES LACKING THE HEAT-STABLE ANTIGEN
SUBPOPULATIONS OF MATURE MURINE THYMOCYTES - PROPERTIES OF CD4-CD8+ AND CD4+CD8- THYMOCYTES LACKING THE HEAT-STABLE ANTIGEN
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DOI：
10.1016/0008-8749(88)90121-9
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发表时间：
1988-12-01
影响因子：
4.3
通讯作者：
SHORTMAN, K
中科院分区：
文献类型：
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作者：
WILSON, A;DAY, LM;SHORTMAN, K
热稳定抗原（HSA），被单克隆抗体M1/69， B2A2和J11d识别，在大多数小鼠外周T细胞表面低或不存在，但在除3%的胸腺细胞外的所有细胞上存在。CD4-CD8+和CD4+CD8-或“单阳性”胸腺群体可根据表面HSA表达进一步分为亚组。一组CD4-CD8+，表达非常高水平的HSA (hsa++)，是不成熟的。T细胞抗原受体（TcR）阴性，外皮层母细胞。然而，CD4-CD8+和CD4+CD8-单阳性可进一步细分为HSA阴性至低（HSA-）和表达适量HSA的（HSA+）。幼鼠胸腺HSA-单阳性比例较低，成年鼠胸腺HSA+单阳性比例相近。成年小鼠单阳性胸腺细胞的HSA-亚群和HSA+亚群均为CD3+，表达正常外周T细胞v . β的发生率。TcR的8个决定因素。在极限稀释培养中，单阳性胸腺细胞的HSA-亚群和HSA+亚群均能产生高频率的增殖克隆，而CD4-CD8+胸腺细胞的HSA-亚群和HSA+亚群克隆均具有细胞毒性。因此HSA-和HSA+单阳性胸腺细胞在功能上都是成熟的。单个阳性胸腺细胞的HSA-亚群与HSA+亚群的不同之处在于，它们的大小略大，表达更高水平的MEL-14，结合更多的花生凝集素，以及包括一部分表达高水平Pgp-1糖蛋白的细胞。这表明HSA- CD4-CD8+和HSA- CD4+CD8-胸腺细胞比它们的HSA+胸腺细胞更成熟，并且可能代表先前激活或“记忆”胸腺亚群。
The heat-stable antigen (HSA), recognized by the monoclonal antibodies M1/69, B2A2, and J11d, is low or absent on the surface of most murine peripheral T cells but present on all but 3% of thymocytes. The CD4-CD8+ and CD4+CD8- or "single positive" thymic populations may be divided into further subgroups based on surface HSA expression. One group, CD4-CD8+ and expressing very high levels of HSA (HSA++), is an immature. T cell antigen receptor (TcR) negative, outer cortical blast cell. However, a further subdivision of CD4-CD8+ and CD4+CD8- single positives may be made, into those negative to low for HSA (HSA-) and those expressing moderate amounts of HSA (HSA+). The proportion of HSA- single positives is low in the thymus of young mice, whereas the proportion of HSA+ single positives is similar to that of the adult. Both the HSA- and the HSA+ subsets of single positive thymocytes from adult mice are CD3+ and express the normal peripheral T cell incidence of V.beta.8 determinants on the TcR. On stimulation with concanavalin A in limit-dilution culture both HSA- and HSA+ subsets of single positive thymocytes give a high frequency of proliferating clones, and the clones from both HSA- and HSA+ subsets of CD4-CD8+ thymocytes are cytotoxic. Thus both HSA- and HSA+ single positive thymocytes are functionally mature. The HSA- subsets of single positive thymocytes differ from the HSA+ subsets in being slightly larger in size, in expressing higher levels of MEL-14, in binding more peanut agglutinin, and in including a proportion of cells expressing high levels of the Pgp-1 glycoprotein. It is suggested that HSA- CD4-CD8+ and HSA- CD4+CD8- thymocytes are more mature than their HSA+ counterparts, and might represent a previously activated or "memory" thymic subpopulation.