Enzymatic cleavage of uracil-containing single-stranded DNA linkers for the efficient release of affinity-selected circulating tumor cells.
Enzymatic cleavage of uracil-containing single-stranded DNA linkers for the efficient release of affinity-selected circulating tumor cells.
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DOI：
10.1039/c4cc09765c
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发表时间：
2015-02-21
期刊：
影响因子：
--
通讯作者：
Soper SA
中科院分区：
文献类型：
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作者：
Nair SV;Witek MA;Jackson JM;Lindell MA;Hunsucker SA;Sapp T;Perry CE;Hupert ML;Bae-Jump V;Gehrig PA;Wysham WZ;Armistead PM;Voorhees P;Soper SA
我们报告了一种新的策略，以高效率（~90%）从表面酶促释放亲和选择的细胞，如循环肿瘤细胞（CTC），同时保持细胞活力（>85%）。该策略利用单链DNA将捕获抗体连接到CTC选择装置的表面。DNA接头含有可被切割的尿嘧啶残基。
We report a novel strategy to enzymatically release affinity-selected cells, such as circulating tumor cells (CTCs), from surfaces with high efficiency (~90%) while maintaining cell viability (>85%). The strategy utilizes single-stranded DNAs that link a capture antibody to the surfaces of a CTC selection device. The DNA linkers contain a uracil residue that can be cleaved.