Rice SST Variation Shapes the Rhizosphere Bacterial Community, Conferring Tolerance to Salt Stress through Regulating Soil Metabolites.
Rice SST Variation Shapes the Rhizosphere Bacterial Community, Conferring Tolerance to Salt Stress through Regulating Soil Metabolites.
复制标题
水稻海温变化塑造根际细菌群落，通过调节土壤代谢物赋予对盐胁迫的耐受性
DOI：
10.1128/msystems.00721-20
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发表时间：
2020-11-24
期刊：
影响因子：
6.4
通讯作者：
Jin J
中科院分区：
文献类型：
--
作者：
Lian T;Huang Y;Xie X;Huo X;Shahid MQ;Tian L;Lan T;Jin J
土壤盐渍化是限制作物产量的主要环境胁迫之一。农业生态系统中的作物已经发展了各种策略来适应盐胁迫。我们使用水稻突变体和CRISPR编辑的品系来研究鳞状细胞启动子结合蛋白盒（SBP box）家族基因（SST/OsSPL 10）、土壤代谢物和根际细菌群落之间的关系。我们发现在盐胁迫下，研究结果为揭示植物根际微生物关键基因在形成根际微生物群落中的作用及其与土壤代谢产物的关系提供了一个有用的范例，并为从微生物和土壤代谢产物角度提高水稻耐高盐能力提供了新的思路。生态视角。摘要某些植物特有的抗病基因可以通过调控根分泌物的释放来影响根际微生物。在先前的研究中，水稻（Oryza sativa）中的SST（幼苗耐盐）基因被鉴定，SST功能的丧失导致植物对盐胁迫的更好适应。然而，水稻SST变化是否可以通过调节根际土壤代谢产物和微生物群来缓解盐胁迫仍不清楚。在这里，我们使用了通过CRISPR/Cas9系统在黄花占（HHZ）和中花11（ZH 11）品种中编辑的具有SST的转基因植物，发现SST功能的丧失增加了水稻植物中钾离子的积累并减少了钠离子的积累。利用16 S rRNA基因扩增子高通量测序，我们发现突变体材料在无盐胁迫下改变了根际细菌的组装。重要的是，在盐胁迫下，sst，HHZcas和ZH 11 cas植物显着改变了根际细菌的组装。此外，水稻SST基因对土壤代谢产物也有影响，这与根际微生物群落的动态密切相关，进一步确定了根际微生物与土壤代谢产物之间的关系。总体而言，我们的研究结果表明，水稻SST基因对盐胁迫的响应与根际土壤微生物群和代谢产物的影响。本研究揭示了水稻SST基因、土壤代谢产物和根际细菌群落组装之间的有益联系，也为通过土壤微生物管理措施改善作物适应性提供了理论依据。重要性土壤盐碱化是限制作物生产力的主要环境胁迫之一。农业生态系统中的作物已经发展了各种策略来适应盐胁迫。我们使用水稻突变体和CRISPR编辑的品系来研究鳞状细胞启动子结合蛋白盒（SBP box）家族基因（SST/OsSPL 10）、土壤代谢物和根际细菌群落之间的关系。我们发现在盐胁迫下，研究结果为揭示植物根际微生物关键基因在形成根际微生物群落中的作用及其与土壤代谢产物的关系提供了一个有用的范例，并为从微生物和土壤代谢产物角度提高水稻耐高盐能力提供了新的思路。生态视角。
Soil salinization is one of the major environmental stresses limiting crop productivity. Crops in agricultural ecosystems have developed various strategies to adapt to salt stress. We used rice mutant and CRISPR-edited lines to investigate the relationships among the Squamosa promoter Binding Protein box (SBP box) family gene (SST/OsSPL10), soil metabolites, and the rhizosphere bacterial community. We found that during salt stress, there are significant differences in the rhizosphere bacterial community and soil metabolites between the plants with the SST gene and those without it. Our findings provide a useful paradigm for revealing the roles of key genes of plants in shaping rhizosphere microbiomes and their relationships with soil metabolites and offer new insights into strategies to enhance rice tolerance to high salt levels from microbial and ecological perspectives. ABSTRACT Some plant-specific resistance genes could affect rhizosphere microorganisms by regulating the release of root exudates. In a previous study, the SST (seedling salt tolerant) gene in rice (Oryza sativa) was identified, and loss of SST function resulted in better plant adaptation to salt stress. However, whether the rice SST variation could alleviate salt stress via regulating soil metabolites and microbiota in the rhizosphere is still unknown. Here, we used transgenic plants with SST edited in the Huanghuazhan (HHZ) and Zhonghua 11 (ZH11) cultivars by the CRISPR/Cas9 system and found that loss of SST function increased the accumulation of potassium and reduced the accumulation of sodium ions in rice plants. Using 16S rRNA gene amplicon high-throughput sequencing, we found that the mutant material shifted the rhizobacterial assembly under salt-free stress. Importantly, under salt stress, the sst, HHZcas, and ZH11cas plants significantly changed the assembly of the rhizobacteria. Furthermore, the rice SST gene also affected the soil metabolites, which were closely related to the dynamics of rhizosphere microbial communities, and we further determined the relationship between the rhizosphere microbiota and soil metabolites. Overall, our results show the effects of the rice SST gene on the response to salt stress associated with the soil microbiota and metabolites in the rhizosphere. This study reveals a helpful linkage among the rice SST gene, soil metabolites, and rhizobacterial community assembly and also provides a theoretical basis for improving crop adaptation through soil microbial management practices. IMPORTANCE Soil salinization is one of the major environmental stresses limiting crop productivity. Crops in agricultural ecosystems have developed various strategies to adapt to salt stress. We used rice mutant and CRISPR-edited lines to investigate the relationships among the Squamosa promoter Binding Protein box (SBP box) family gene (SST/OsSPL10), soil metabolites, and the rhizosphere bacterial community. We found that during salt stress, there are significant differences in the rhizosphere bacterial community and soil metabolites between the plants with the SST gene and those without it. Our findings provide a useful paradigm for revealing the roles of key genes of plants in shaping rhizosphere microbiomes and their relationships with soil metabolites and offer new insights into strategies to enhance rice tolerance to high salt levels from microbial and ecological perspectives.