A carbon sandwich environmental cell for wet specimens

A carbon sandwich environmental cell for wet specimens
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用于湿样品的碳夹层环境池

DOI：
10.1093/jmicro/dft027
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发表时间：
2013
期刊：
Microscopy
影响因子：
1.8
通讯作者：
Y. Inayoshi and H.Minoda
Y. Inayoshi and H.Minoda
中科院分区：
工程技术4区
文献类型：
--
作者：
H. Mouri;A. Hori;Y. Kawashima; K. Hashimoto;松田裕一,西村拓史,藪博之;Y. Inayoshi and H.Minoda

文献摘要

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研制了一种用于环境透射电子显微镜（E-TEM）的湿样品碳夹层环境池。用一种有许多孔的塑料薄膜制成碳胶囊。碳夹层环境池由隔板和两层碳膜组成，将样品和实验溶液封装在其中。可以通过改变用于制备塑料间隔物的条件来控制水层的厚度。与我们之前的E-TEM相比，图像质量得到了提高，E-TEM可以在样品周围循环气体。通过使用松散凝聚的DNA获得了2nm的分辨率。
A carbon sandwich environmental cell for wet specimens was developed for environmental transmission electron microscopy (E-TEM). A plastic film with many holes was used to form carbon capsules. The carbon sandwich environmental cells were composed of the spacer and two carbon films and enclosing the samples and experimental solution. The thickness of the water layer can be controlled by changing the conditions used to prepare the plastic spacers. The quality of the images was improved over our previous E-TEM, which could circulate gas around samples. A resolution of 2 nm was obtained by using loosely condensed DNAs.