Cell surface galactosyltransferase acts as a general modulator of rat acinar cell proliferation.
Cell surface galactosyltransferase acts as a general modulator of rat acinar cell proliferation.
复制标题
细胞表面半乳糖基转移酶充当大鼠腺泡细胞增殖的一般调节剂。
DOI：
10.1007/bf00219524
复制
发表时间：
1990
影响因子：
4.3
通讯作者：
Schneyer,CA
中科院分区：
文献类型：
--
作者：
Humphreys-Beher,MG;Zelles,T;Maeda,N;Purushotham,KR;Schneyer,CA
检查了几个生理参数以诱导腺泡细胞增殖和相应的 β1-4 半乳糖基转移酶表达增加。在这项研究中，导致腺泡细胞增殖的饮食变化包括以下内容：给动物引入流质饮食（导致腺体萎缩），然后重新引入固体食物，通过在动物饮用水中加入0.5%柠檬酸来提供味觉刺激，以及去除下颌下腺，随后依赖腮腺获取唾液蛋白。通过给动物注射神经生长因子或表皮生长因子的长期（3天）方案来改变生长因子水平。除 EGF 外，通过注射普萘洛尔（一种 β-肾上腺素受体拮抗剂）或半乳糖基转移酶特异性修饰蛋白 α-乳白蛋白，所有情况下均可阻断腮腺增殖。然而，可以通过用 EGF 受体单克隆抗体或半乳糖基转移酶修饰蛋白 a-乳清蛋白治疗动物来预防 EGF 诱导的增殖。正常腺泡细胞增殖的这些结果表明细胞表面 β1-4 半乳糖基转移酶在信号传导和维持活跃的细胞生长中具有直接作用。
Several physiological parameters were examined for inducing acinar cell proliferation and corresponding increased expression of β1–4 galactosyltransferase. In this study, dietary changes causing acinar cell proliferation included the following: the introduction of animals to a liquid diet (causing gland atrophy) followed by reintroduction of solid chow, gustatory stimulation provided by the introduction of 0.5% citric acid to animal drinking water, and removal of the submandibular gland with subsequent reliance on the parotid gland for salivary protein. Alterations in growth factor levels were produced by injecting animals with a chronic (3 day) regimen of either nerve growth factor or epidermal growth factor. Parotid gland proliferation could be blocked in all cases except EGF by the injection of propranolol, a β-adrenoceptor antagonist, or the galactosyltransferase specific modifier protein, α-lactalbumin. EGF-induced proliferation could, however, be prevented by treating the animals with monoclonal antibody to EGF receptor or galactosyltransferase modifier protein a-lactalbumin. These results for normal acinar cell proliferation suggest a direct role for cell surface β1–4 galactosyltransferase in signalling and maintaining active cell growth.