Maize transcription factor ZmNF-YC13 regulates plant architecture
Maize transcription factor ZmNF-YC13 regulates plant architecture
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玉米转录因子 ZmNF-YC13 调节植物结构
DOI：
10.1093/jxb/erab157
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发表时间：
2021
影响因子：
6.9
通讯作者：
Yilin Cai
中科院分区：
文献类型：
--
作者：
Xiupeng Mei;Jin Nan;Zikun Zhao;Shun Yao;Wenqin Wang;Yang Yang;Yang Bai;Erfei Dong;Chaoxian Liu;Yilin Cai
叶角和叶向值是决定玉米株型的重要农艺性状。核因子Y（NF-Y）成员在植物构型调控中的功能尚未见报道。ZmNF-YC 13在玉米叶片基部高表达，ZmNF-YC 13过表达植株叶片直立，叶角较窄，LOV较大，ZmNF-YC 13敲除植株叶角较大，LOV较小。植物结构的变化是由于细胞色素P450家族成员表达的变化。ZmNF-YC 13与叶子叶1亚家族的两个NF-Y亚基B成员（ZmNF-YB 9和ZmNF-YB 10）相互作用，并进一步募集NF-Y亚基A（ZmNF-YA 3）以形成两个NF-Y复合物。这两种复合物均能激活转录抑制子ZmWRKY 76和ZmBT 2的启动子，其中PLASTOCHRON组基因的启动子能被ZmWRKY 76和ZmBT 2抑制。ZmNF-YC 13与ZmNF-YBs和ZmNF-YA 3一起，通过调控ZmWRKY 76和ZmBT 2的表达，抑制质体染色体分支中细胞色素P450家族成员的表达，从而影响植物的结构。
Leaf angle and leaf orientation value (LOV) are critical agronomic traits for maize plant architecture. The functions of NUCLEAR FACTOR Y (NF-Y) members in regulating plant architecture have not been reported yet. Here, we identified a regulator of maize plant architecture, NF-Y subunit C13 (ZmNF-YC13).ZmNF-YC13was highly expressed in the leaf base zone of maize plants.ZmNF-YC13overexpressing plants showed upright leaves with narrow leaf angle and larger LOV, whileZmNF-YC13knockout plants had larger leaf angle and smaller LOV compared with wild-type plants. The changes in plant architecture were due to the changes in the expression of cytochrome P450 family members. ZmNF-YC13 interacts with two NF-Y subunit B members (ZmNF-YB9 and ZmNF-YB10) of the LEAFY COTYLEDON1 sub-family, and further recruits NF-Y subunit A (ZmNF-YA3) to form two NF-Y complexes. The two complexes can both activate the promoters of transcriptional repressors (ZmWRKY76 and ZmBT2), and the promoters ofPLASTOCHRONgroup genes can be repressed by ZmWRKY76 and ZmBT2 in maize protoplasts. We propose that ZmNF-YC13 functions as a transcriptional regulator and, together with ZmNF-YBs and ZmNF-YA3, affects plant architecture by regulating the expression ofZmWRKY76andZmBT2, which repress the expression of cytochrome P450 family members inPLASTOCHRONbranch.