Modified messenger ribonucleic acid release from isolated hepatic nuclei after inhibition of polyadenylate formation.
Modified messenger ribonucleic acid release from isolated hepatic nuclei after inhibition of polyadenylate formation.
复制标题
抑制聚腺苷酸形成后，修饰的信使核糖核酸从分离的肝细胞核中释放。
作者：
D. Schumm;T. E. Webb
用虫草素（3 '-脱氧腺苷）（一种核多聚腺苷酸合成的抑制剂）处理大鼠，可选择性地降低无细胞系统中离体肝细胞核中部分mRNA种类的能量和细胞溶质依赖性释放约40%。对含有poly（A）束的转运mRNA与硝酸纤维素过滤器以及纤维素或poly（U）-琼脂糖柱的差异结合的分析表明，从虫草素处理的大鼠的分离肝核转运的mRNA中的poly（A）束的大小减小。通过分析聚（A）束的平均大小，从信使核糖核酸酶活性，蔗糖密度梯度上释放的大小减少得到证实。
Treatment of rats with Cordycepin (3'-deoxyadenosine), an inhibitor of nuclear poly(A) synthesis, selectively decreased the energy and cytosol-dependent release of a portion of the mRNA species from the isolated hepatic nuclei in a cell-free system by approximately 40%. An analysis of the differential binding of the transported mRNA containing poly(A) tracts to nitrocellulose filters, and to cellulose or poly(U)-Sepharose columns suggested that the poly(A) tracts in the mRNA transported from the isolated hepatic nuclei of Cordycepin-treated rats, are decreased in size. This size decrease was confirmed through an analysis of the average size of the poly(A) tracts, released from the messengers by ribonuclease activity, on sucrose density gradients.