Lymphocyte migration in the micro-channel of splenic sheathed capillaries in Chinese soft-shelled turtles, Pelodiscus sinensis
Lymphocyte migration in the micro-channel of splenic sheathed capillaries in Chinese soft-shelled turtles, Pelodiscus sinensis
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中华鳖脾毛细血管微通道中淋巴细胞的迁移
DOI：
10.1016/j.micron.2015.09.003
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发表时间：
2016-01-01
期刊：
影响因子：
2.4
通讯作者：
Chen, Qiusheng
中科院分区：
文献类型：
--
作者：
Zhang, Qian;Ullah, Shakeeb;Chen, Qiusheng
用光学显微镜和透射电子显微镜观察了脾鞘毛细血管的结构特征。本研究主要研究中华鳖脾白髓中淋巴细胞通过微通道向脾白髓的迁移。结果表明，甲鱼脾内有鞘的毛细血管为高内皮微静脉样血管。这些毛细血管由促进淋巴细胞向脾白髓迁移的微通道组成。微通道是由内皮细胞、支持细胞和椭球相关细胞组成的动态结构，为淋巴细胞的迁移提供了微环境。甲鱼脾微通道中淋巴细胞的迁移模式包括以下几个步骤：(1)淋巴细胞首先附着在有鞘毛细血管的内皮上，穿过内皮细胞，穿过有鞘毛细血管的基底膜；(2)然后与支持细胞和EAC结合进入椭球；(3)淋巴细胞通过微通道从椭圆形迁移到椭圆形周围淋巴鞘(PELS)。本研究为淋巴细胞在甲鱼脾微通道内的迁移提供了形态学证据，并对爬行动物脾白髓淋巴细胞归巢的机制进行了深入的探讨。(C)2015爱思唯尔有限公司。保留所有权利。
The structural characteristics of the splenic sheathed capillary were investigated using light microscopy and transmission electron microscopy (TEM). This study mainly focused on lymphocyte migration to the splenic white pulp via micro-channels in Chinese soft-shelled turtles, Pelodiscus sinensis. The results showed that the sheathed capillaries in the turtle spleen were high endothelial venule (HEV)-like vessels. These capillaries consist of micro-channels that facilitate lymphocyte migration to the splenic white pulp. The micro-channel is a dynamic structure comprising processes of endothelial cells, supporting cells, and ellipsoid-associated cells (EACs), which provides a microenvironment for lymphocyte migration. The pattern of lymphocyte migration in the micro-channel of the turtle spleen includes the following steps: (i) lymphocyte first adheres to the endothelium of the sheathed capillary, passes through the endothelial cells, and traverses through the basement membrane of the sheathed capillary; (ii) it then enters into the ellipsoid combined with supporting cells and EACs; and (iii) lymphocyte migrates from the ellipsoid to the periellipsoidal lymphatic sheath (PELS) via the micro-channel. This study provides morphological evidence for lymphocyte migration in the micro-channels of turtle spleens and also an insight into the mechanism of lymphocyte homing to the splenic white pulp of reptiles. (C) 2015 Elsevier Ltd. All rights reserved.