Expression and purification of functional HMGB1 A box by fusion with SUMO.
Expression and purification of functional HMGB1 A box by fusion with SUMO.
复制标题
通过与 SUMO 融合表达和纯化功能性 HMGB1 A 盒。
DOI：
10.3892/mmr.2015.4308
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发表时间：
2015-11
影响因子：
3.4
通讯作者：
Xu LM
中科院分区：
文献类型：
--
作者：
Ge WS;Fan JG;Chen YW;Xu LM
高迁移率族盒染色体蛋白1(HMGB1)是真核细胞中普遍存在且含量丰富的核蛋白。核HMGB1在维持应激状态下的核稳定方面发挥着重要作用。然而，细胞外HMGB1发挥的作用与细胞内的这些功能明显不同。当HMGB1在细胞外释放时，它是一种强大的天然信号，启动宿主防御机制或组织再生。HMGB1有两个DNA结合域：HMG A盒和B盒。HMGB1A盒具有拮抗、抗炎作用，是一种潜在的治疗靶点，但HMGB1A盒的高效大规模表达和纯化仍未见报道。本研究利用相扑融合表达系统表达和纯化高水平的功能性HMGB1A盒，以满足治疗性蛋白生产的需要。
High-mobility-group-box chromosomal protein 1 (HMGB1) is a ubiquitous and abundant nuclear protein in eukaryotic cells. Nuclear HMGB1 serves an important role in maintaining nuclear stability under stress. However, extracellular HMGB1 exerts actions, which are distinctly different compared with these intracellular functions. HMGB1, when released extracellularly, is a potent innate signal, which initiates host defense mechanisms or tissue regeneration. HMGB1 has two DNA-binding domains: HMG A box and B box. The HMGB1 A box exhibits an antagonistic, anti-inflammatory effect, and is a potential therapeutic target, however, the large-scale expression and purification of the HMGB1 A box with high efficiency remains to be reported. In the present study, a SUMO-fusion expression system was used to express and purify high levels of functional HMGB1 A box to meet the requirements of therapeutic protein production.