Aspergillus SteA (Sterile12‐like) is a homeodomain‐C2/H2‐Zn+2 finger transcription factor required for sexual reproduction
Aspergillus SteA (Sterile12‐like) is a homeodomain‐C2/H2‐Zn+2 finger transcription factor required for sexual reproduction
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DOI：
10.1046/j.1365-2958.2000.01874.x
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发表时间：
2000-04
影响因子：
3.6
通讯作者：
M. Vallim;K. Miller;B. Miller
中科院分区：
文献类型：
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作者：
M. Vallim;K. Miller;B. Miller
酿酒酵母（Saccharomyces cerevisiae）Ste 12 p在通过MAP激酶模块将信号转导偶联到交配和假菌丝（PH）/丝状生长（FG）所需的细胞特异性或形态发生特异性基因表达中起关键作用。致病性酵母菌白色念珠菌和Filobasidiela neoformans中的Ste 12 p同源物在二态转变过程中显然起着类似的作用。在这里，我们报告的第一个Ste 12蛋白从一个真正的丝状真菌的分离和鉴定。构巢曲霉steA编码的蛋白与其他Ste 12蛋白的同源结构域具有63-75%的相同性，与FnSte 12 αp的相似性最大。SteAp和Ste 12 αp缺乏在芽殖酵母Ste 12 p中发现的信息素诱导结构域，但具有在其他Ste 12蛋白中不存在的C末端C2/H2-Zn+2指状结构域。ΔsteA菌株是不育的，并且既不区分子囊组织也不区分子实体（闭囊酵母）。然而，性周期特异性Hülle细胞的发育不受影响。在缺失菌株中，丝状菌生长、分生孢子和PH样无性生殖细胞（代谢芽和瓶梗）的分化是正常的。北方的无性和有性生殖周期的关键调节因子的分析支持的观察，虽然SteAp功能仅限于性周期，两个发育途径之间的交叉调节存在。我们的研究结果进一步表明，虽然几类相关蛋白控制着A。在nidulans和二型酵母中，在调节回路中必须存在显著差异。
Saccharomyces cerevisiae Ste12p plays a key role in coupling signal transduction through MAP kinase modules to cell‐specific or morphogenesis‐specific gene expression required for mating and pseudohyphal (PH)/filamentous growth (FG). Ste12p homologues in the pathogenic yeasts Candida albicans and Filobasidiela neoformans apparently play similar roles during dimorphic transitions. Here we report the isolation and characterization of the first Ste12 protein from a true filamentous fungus. Aspergillus nidulans steA encodes a protein with a homeodomain 63–75% identical to those of other Ste12 proteins, with greatest similarity to FnSte12αp. SteAp and Ste12αp lack the pheromone induction domain found in budding yeast Ste12p, but have C‐terminal C2/H2‐Zn+2 finger domains not present in the other Ste12 proteins. A ΔsteA strain is sterile and differentiates neither ascogenous tissue nor fruiting bodies (cleistothecia). However, the development of sexual cycle‐specific Hülle cells is unaffected. Filamentous growth, conidiation and the differentiation of PH‐like asexual reproductive cells (metulae and phialides) are normal in the deletion strain. Northern analysis of key regulators of the asexual and sexual reproductive cycles support the observation that although SteAp function is restricted to the sexual cycle, cross regulation between the two developmental pathways exists. Our results further suggest that while several classes of related proteins control similar morphogenetic events in A. nidulans and the dimorphic yeasts, significant differences must exist in the regulatory circuitry.