In vivo validation of a bimodal technique combining time-resolved fluorescence spectroscopy and ultrasonic backscatter microscopy for diagnosis of oral carcinoma
In vivo validation of a bimodal technique combining time-resolved fluorescence spectroscopy and ultrasonic backscatter microscopy for diagnosis of oral carcinoma
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DOI：
10.1117/1.jbo.17.11.116003
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发表时间：
2012-11-01
影响因子：
3.5
通讯作者：
Marcu, Laura
中科院分区：
文献类型：
--
作者：
Sun, Yang;Xie, Hongtao;Marcu, Laura
组织诊断功能所产生的双峰技术集成扫描时间分辨荧光光谱（TRFS）和超声背散射显微镜（UBM）在体内仓鼠口腔癌模型进行了研究。组织荧光由脉冲氮激光激发，并分别使用一组滤波器/分色镜和快速数字化仪进行光谱和时间分辨。一个41 MHz的聚焦换能器（37 μ m的轴向，65 μ m的横向分辨率）用于UBM扫描。癌变不同阶段的代表性病变显示荧光特征与超声特征互补，两者均与组织学发现相关。这些结果表明，TRFS-UBM提供了丰富的共同注册，补充数据的组织组成和结构，因为它涉及到疾病状态。TRFS数据（对表面分子变化敏感）与UBM数据（对横截面结构变化和肿瘤浸润深度敏感）的直接配准有望在术前诊断和术中确定肿瘤边缘中发挥重要作用。(c)2012年，美国光电仪器工程师学会（SPIE）。[DOI：10.1117/1.JBO.17.11.116003]
Tissue diagnostic features generated by a bimodal technique integrating scanning time-resolved fluorescence spectroscopy (TRFS) and ultrasonic backscatter microscopy (UBM) are investigated in an in vivo hamster oral carcinoma model. Tissue fluorescence is excited by a pulsed nitrogen laser and spectrally and temporally resolved using a set of filters/dichroic mirrors and a fast digitizer, respectively. A 41-MHz focused transducer (37-mu m axial, 65-mu m lateral resolution) is used for UBM scanning. Representative lesions of the different stages of carcinogenesis show that fluorescence characteristics complement ultrasonic features, and both correlate with histological findings. These results demonstrate that TRFS-UBM provide a wealth of co-registered, complementary data concerning tissue composition and structure as it relates to disease status. The direct co-registration of the TRFS data (sensitive to surface molecular changes) with the UBM data (sensitive to cross-sectional structural changes and depth of tumor invasion) is expected to play an important role in pre-operative diagnosis and intra-operative determination of tumor margins. (c) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.11.116003]