Protective Effect of Koumine, an Alkaloid from Gelsemium Sempervirens, on Injury Induced by H2O2 in IPEC-J2 Cells
Protective Effect of Koumine, an Alkaloid from Gelsemium Sempervirens, on Injury Induced by H2O2 in IPEC-J2 Cells
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钩吻生物碱 Koumine 对 H2O2 诱导的 IPEC-J2 细胞损伤的保护作用
DOI：
10.3390/ijms20030754
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发表时间：
2019
影响因子：
5.6
通讯作者：
Sun Zhiliang
中科院分区：
文献类型：
--
作者：
Yuan Zhihang;Liang Zengenni;Yi Jine;Chen Xiaojun;Li Rongfang;Wu Yong;Wu Jing;Sun Zhiliang
草药植物在世界范围内被广泛用于干预不同的疾病和改善健康。钩吻是一种广泛存在于钩吻植物中的生物碱单体，可以有效地用作抗氧化剂。本研究旨在评价口服液对过氧化氢(H_2O_2)诱导的猪肠上皮细胞株(IPEC-J2细胞)氧化应激和细胞凋亡的保护作用。四甲基偶氮唑盐比色法显示，钩吻素能显著提高过氧化氢诱导的IPEC-J2细胞的存活率。钩吻素预孵育可通过减少活性氧(ROS)的产生，有效地抑制乳酸脱氢酶(LDH)的释放和丙二醛(MDA)的产生，从而减轻H_2O_2诱导的细胞凋亡。此外，在过氧化氢诱导的IPEC-J2细胞中，超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽(GSH)活性在库明作用下恢复到正常水平。此外，库明可抑制H_2O_2诱导的线粒体膜电位下降、caspase-9和caspase-3的激活、bc l-2表达的降低和Bax表达的升高。综上所述，本研究结果表明，Koumine通过抑制ROS的产生，抑制caspase-3的活性，影响Bax和Bcl2的表达，对H_2O_2作用下的IPEC-J2细胞具有保护作用。Koumine对过氧化氢诱导的细胞凋亡有潜在的保护作用。
Medicinal herbal plants have been commonly used for intervention in different diseases and improvement of health worldwide. Koumine, an alkaloid monomer found abundantly in Gelsemium plants, can be effectively used as an antioxidant. The purpose of this study was to evaluate the potential protective effect of koumine against hydrogen peroxide (H2O2)-induced oxidative stress and apoptosis in porcine intestinal epithelial cell line (IPEC-J2 cells). MTT assays showed that koumine significantly increased cell viability in H2O2-mediated IPEC-J2 cells. Preincubation with koumine ameliorated H2O2-medicated apoptosis by decreasing reactive oxygen species (ROS) production, and efficiently suppressed the lactate dehydrogenase (LDH) release and malondialdehyde (MDA) production. Moreover, a loss of superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) activities was restored to normal level in H2O2-induced IPEC-J2 cells upon koumine exposure. Furthermore, pretreatment with koumine suppressed H2O2-mediated loss of mitochondrial membrane potential, caspase-9 and caspase-3 activation, decrease of Bcl-2 expression and elevation of Bax expressions. Collectively, the results of this study indicated that koumine possesses the cytoprotective effects in IPEC-J2 cells during exposure to H2O2 by suppressing production of ROS, inhibiting the caspase-3 activity and influencing the expression of Bax and Bcl-2. Koumine could potentially serve as a protective effect against H2O2-induced apoptosis.