Facilitated magnetic resonance imaging diagnosis of pulmonary disease using a macromolecular blood-pool contrast agent, polylysine-(Gd-DTPA)40.
Facilitated magnetic resonance imaging diagnosis of pulmonary disease using a macromolecular blood-pool contrast agent, polylysine-(Gd-DTPA)40.
复制标题
使用大分子血池造影剂聚赖氨酸-(Gd-DTPA)40 促进肺部疾病的磁共振成像诊断。
DOI：
10.1097/00004424-199111001-00013
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发表时间：
1991
影响因子：
6.7
通讯作者：
Jerome,H
中科院分区：
文献类型：
--
作者：
Brasch,RC;Berthezene,Y;Vexler,VS;Moseley,M;Clément,O;Muehler,A;Price,D;Jerome,H
雌性Sprague-Dawley大鼠（225-250 g）通过腹膜内给予戊巴比妥钠（40-50 mg/kg）麻醉。将23号蝶形插管插入尾静脉，并用无菌盐水冲洗。通过中线颈部切口，将聚乙烯套管插入气管中用于辅助通气（哈佛小动物呼吸器，米利斯，MA）。呼吸频率维持在75次/min，潮气量为0.5mL。12只仰卧位大鼠静脉注射油酸诱导毛细血管渗漏性肺水肿（0.075mL/kg），以0.9%氯化钠持续静脉滴注造成大鼠静水压性水肿（20-30分钟内体重的25%），在注射油酸后30分钟或完成生理盐水输注后立即进行MRI。
Female Sprague-Dawley rats (225–250 g) were anesthetized by intraperitoneal administration of sodium pentobarbital (40–50 mg/kg). A 23-gauge butterfly cannula was inserted in a tail vein and flushed with sterile saline. Through a midline cervical incision, a polyethylene cannula was inserted into the trachea for assisted ventilation (Harvard small animal respirator, Millis, MA). The respiratory rate was maintained at 75 breaths per minute, with a tidal volume of 0.5 mL.Capillary leak pulmonary edema was induced in 12 supine rats by venous injection of oleic acid (0.075 mL/kg), Hydrostatic edema was induced in ten supine rats by continuous intravenous infusion of 0.9% sodium chloride (25% of body weight over 20–30 minutes), MRI was performed either 30 minutes after injection of oleic acid or immediately after the completion of saline infu-