Methylation Profiles in Breast Cancer
Methylation Profiles in Breast Cancer
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DOI：
10.1080/07357900802350814
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发表时间：
2009-01-01
影响因子：
2.4
通讯作者：
Dalay, Nejat
中科院分区：
文献类型：
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作者：
Buyru, Nur;Altinisik, Julide;Dalay, Nejat
在这项研究中，我们试图通过使用甲基化特异性多重连接依赖探针扩增来评估单一反应中多个肿瘤抑制基因的异常甲基化。对77例乳腺肿瘤及相应的正常组织进行分析。在这项研究中，24个基因中的17个在一个或多个肿瘤样本中显示启动子甲基化。最常见的甲基化基因是RASSF1和GSTP1，其次是DAPK1和CDKN2B。我们的数据表明，特定基因的甲基化在乳腺癌中是一个常见的事件，并表明MS-MLPA是一个强大的工具，用于分析诊断和治疗目的的表观遗传改变。
In this study, we sought to assess the aberrant methylation of multiple tumor-suppressor genes in a single reaction by using methylation-specific multiplex ligation-dependent probe amplification. Breast tumors and corresponding normal tissues of 77 patients were analyzed. In this study, 17 of 24 genes displayed promoter methylation in one or more of the tumor samples. The most frequently methylated genes were RASSF1 and GSTP1, followed by DAPK1 and CDKN2B. Our data indicate that the methylation of specific genes is a frequent event in breast cancer and show that MS-MLPA is a powerful tool to analyze epigenetic alterations for diagnostic, as well as therapeutic, purposes.