PLC zeta: a sperm-specific trigger of Ca(2+) oscillations in eggs and embryo development.
PLC zeta: a sperm-specific trigger of Ca(2+) oscillations in eggs and embryo development.
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发表时间：
2002-08
期刊：
影响因子：
4.6
通讯作者：
C. Saunders;M. Larman;J. Parrington;L. J. Cox;J. Royse;L. Blayney;K. Swann;F. Lai
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作者：
C. Saunders;M. Larman;J. Parrington;L. J. Cox;J. Royse;L. Blayney;K. Swann;F. Lai
在精子受精后，哺乳动物卵子被一系列细胞内Ca（2+）振荡激活，这对胚胎发育至关重要。精子诱导这种复杂信号现象的机制尚不清楚。有一种观点认为，精子将一种独特的胞质因子引入卵子，促使Ca（2+）释放。“精子因子”假说还没有得到证实，因为还没有发现一种精子特异性蛋白质，它能产生重复的Ca（2+）瞬变和卵子激活。我们鉴定了一种新的精子特异性磷脂酶C，PLC zeta，它能触发小鼠卵子中的Ca（2+）振荡，与受精时的Ca（2+）振荡难以区分。从精子提取物中去除PLC zeta可消除卵子中的Ca（2+）释放。此外，单个精子的PLC zeta含量足以产生Ca（2+）振荡以及正常的胚胎发育到囊胚。我们的研究结果与精子PLC zeta作为受精卵发育成胚胎的分子触发器是一致的。
Upon fertilisation by sperm, mammalian eggs are activated by a series of intracellular Ca(2+) oscillations that are essential for embryo development. The mechanism by which sperm induces this complex signalling phenomenon is unknown. One proposal is that the sperm introduces an exclusive cytosolic factor into the egg that elicits serial Ca(2+) release. The 'sperm factor' hypothesis has not been ratified because a sperm-specific protein that generates repetitive Ca(2+) transients and egg activation has not been found. We identify a novel, sperm-specific phospholipase C, PLC zeta, that triggers Ca(2+) oscillations in mouse eggs indistinguishable from those at fertilisation. PLC zeta removal from sperm extracts abolishes Ca(2+) release in eggs. Moreover, the PLC zeta content of a single sperm was sufficient to produce Ca(2+) oscillations as well as normal embryo development to blastocyst. Our results are consistent with sperm PLC zeta as the molecular trigger for development of a fertilised egg into an embryo.