The canine activated platelet secretome (CAPS): A translational model of thrombin-evoked platelet activation response.
The canine activated platelet secretome (CAPS): A translational model of thrombin-evoked platelet activation response.
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DOI：
10.1002/rth2.12450
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发表时间：
2021-01
影响因子：
4.6
通讯作者：
Brooks MB
中科院分区：
文献类型：
--
作者：
Cremer SE;Catalfamo JL;Goggs R;Seemann SE;Kristensen AT;Szklanna PB;Maguire PB;Brooks MB
家犬是研究自然发生的人类疾病的转化动物模型。蛋白质组学已成为表征人类血小板病理生理学的一种有前途的工具;因此，对核心犬活化血小板分泌组（CAPS）的详细表征将提高犬模型的利用率。本研究的目的是开发一种稳健、高通量、无标记的方法，用于犬血小板（i）凝血酶释放蛋白和（ii）构成CAPS的蛋白亚组的蛋白质组学鉴定和定量。从10只健康犬中分离血小板，并用50 nmol/L γ-凝血酶或生理盐水刺激。蛋白质在溶液中胰蛋白酶消化，并通过纳米液相色谱-串联光谱法进行分析。核心释放蛋白定义为存在于10只狗中的10只中的那些，并且CAPS定义为在刺激的相对于盐水对照中具有显著更高丰度的释放蛋白（校正的P <0.05）。共鉴定出2865种蛋白质，其中1126种释放蛋白存在于所有犬中，650种被定义为CAPS。与人血小板的差异包括犬缺乏血小板因子4和血管内皮生长因子C，以及低10至20倍的蛋白质浓度，如结合珠蛋白，α 2巨球蛋白，血管性血友病因子和淀粉样β A4。28种CAPS蛋白，包括细胞因子、粘附分子、颗粒蛋白和钙调节蛋白，以前未被归因于人血小板。CAPS蛋白代表了一种大型动物血小板分泌组的强大表征，也是一种建模血小板生理学、病理生理学和鉴定血小板介导疾病的翻译生物标志物的新工具。
Domestic dogs represent a translational animal model to study naturally occurring human disease. Proteomics has emerged as a promising tool for characterizing human platelet pathophysiology; thus a detailed characterization of the core canine activated platelet secretome (CAPS) will enhance utilization of the canine model. The objectives of this study were development of a robust, high throughput, label‐free approach for proteomic identification and quantification of the canine platelet (i) thrombin releasate proteins, and (ii) the protein subgroup that constitutes CAPS. Platelets were isolated from 10 healthy dogs and stimulated with 50 nmol/L of γ‐thrombin or saline. Proteins were in‐solution trypsin‐digested and analyzed by nano–liquid chromatography–tandem spectrometry. Core releasate proteins were defined as those present in 10 of 10 dogs, and CAPS defined as releasate proteins with a significantly higher abundance in stimulated versus saline controls (corrected P < .05). A total of 2865 proteins were identified; 1126 releasate proteins were present in all dogs, 650 were defined as CAPS. Among the differences from human platelets were a canine lack of platelet factor 4 and vascular endothelial growth factor C, and a 10‐ to 20‐fold lower concentration of proteins such as haptoglobin, alpha‐2 macroglobulin, von Willebrand factor, and amyloid‐beta A4. Twenty‐eight CAPS proteins, including cytokines, adhesion molecules, granule proteins, and calcium regulatory proteins have not previously been attributed to human platelets. CAPS proteins represent a robust characterization of a large animal platelet secretome and a novel tool to model platelet physiology, pathophysiology, and to identify translational biomarkers of platelet‐mediated disease.
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影响因子：
14.9
作者：
Deutsch EW;Csordas A;Sun Z;Jarnuczak A;Perez-Riverol Y;Ternent T;Campbell DS;Bernal-Llinares M;Okuda S;Kawano S;Moritz RL;Carver JJ;Wang M;Ishihama Y;Bandeira N;Hermjakob H;Vizcaíno JA
通讯作者：
Vizcaíno JA
影响因子：
1.3
作者：
Goggs, Robert;Mastrocco, Alicia;Brooks, Marjory B.
通讯作者：
Brooks, Marjory B.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
--
作者：
Defontis, Myriam;Cote, Serge;Ledieu, David
通讯作者：
Ledieu, David
DOI：
10.4049/jimmunol.1102674
发表时间：
2012-06-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Derive M;Bouazza Y;Sennoun N;Marchionni S;Quigley L;Washington V;Massin F;Max JP;Ford J;Alauzet C;Levy B;McVicar DW;Gibot S
通讯作者：
Gibot S