Continuous microwave-assisted step-by-step extraction of bioactive water-soluble materials and fucoidan from brown seaweed Undaria pinnatifida waste
Continuous microwave-assisted step-by-step extraction of bioactive water-soluble materials and fucoidan from brown seaweed Undaria pinnatifida waste
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DOI：
10.1007/s13399-022-03035-6
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发表时间：
2022-07
影响因子：
4
通讯作者：
C. Sasaki;Satoshi Tamura;Miyuki Suzuki;Kanako Etomi;Nobuya Nii;J. Hayashi;K. Kanemaru
中科院分区：
文献类型：
--
作者：
C. Sasaki;Satoshi Tamura;Miyuki Suzuki;Kanako Etomi;Nobuya Nii;J. Hayashi;K. Kanemaru
研究了微波辅助提取法（MAE）在低温度（55 ~ 160℃）和高温度（150 ~ 170℃）下对褐藻废弃物的连续提取工艺，以获得蛋白质和硫酸酸化多糖岩藻聚糖。在较低温度下，蛋白质提取率随提取条件严酷程度的增加而增加。所有提取的蛋白均表现出抑制血管紧张素转换酶的活性。分别在100°C和120°C下提取20和5 min的蛋白活性最高（以蛋白浓度的ic50评估），分别为0.03和0.05 g/L。随后，在高温提取过程中，在150°C下提取30分钟（根据原料的12.3%）后获得了最高的岩藻糖聚糖收率。产率与直接经MAE处理（不经低温处理）提取的产率相当。结果表明，用MAE连续提取蛋白质和岩藻聚糖是可行的。此外,U。裙带鱼废弃物可以作为原料，提供有价值的化学品。
Continuous extraction production process by microwave-assisted extraction (MAE) using brown macroalgaeUndaria pinnatifidawaste was investigated to obtain proteins and a sulfated polysaccharide, fucoidan, at low (55–160 °C) and high (150–170 °C) temperatures. At the lower temperatures, protein extraction increased with increasing extraction condition severity. All extracted proteins exhibited angiotensin-converting enzyme inhibitory activity. The proteins extracted at 100 and 120 °C for 20 and 5 min, respectively, displayed the highest activities (evaluated as IC50of protein concentration), which were 0.03 and 0.05 g/L, respectively. Subsequently, the highest fucoidan yields were attained after extraction at 150 °C for 30 min (12.3% based on the raw material) during the higher temperature extraction process. The yield was equivalent to that extracted directly by the MAE treatment (without treatment at a lower temperature). The results indicated that the continuous extraction of protein and fucoidan using MAE is possible. Moreover,U. pinnatifidawaste could potentially be used as feedstock to provide valuable chemicals.