Oral SARS-CoV-2 Inoculation Establishes Subclinical Respiratory Infection with Virus Shedding in Golden Syrian Hamsters.
Oral SARS-CoV-2 Inoculation Establishes Subclinical Respiratory Infection with Virus Shedding in Golden Syrian Hamsters.
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DOI：
10.1016/j.xcrm.2020.100121
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发表时间：
2020-10-20
期刊：
影响因子：
--
通讯作者：
Yuen KY
中科院分区：
文献类型：
--
作者：
Lee AC;Zhang AJ;Chan JF;Li C;Fan Z;Liu F;Chen Y;Liang R;Sridhar S;Cai JP;Poon VK;Chan CC;To KK;Yuan S;Zhou J;Chu H;Yuen KY
严重急性呼吸综合征-冠状病毒2(SARS-CoV-2)主要通过呼吸道飞沫或空气中的气雾剂传播。尽管经常在患者的直接环境和粪便中发现SARS-CoV-2，但没有证据支持口服感染SARS-CoV-2。利用叙利亚仓鼠模型，我们证明了鼻内吸入SARS-CoV-2引起的肺炎的严重性随着病毒接种量的增加而增加。SARS-CoV-2在体外模拟人胃液和空腹肠液中接种2小时后仍保持感染性，经鼻接种最高接种量(105PFU)的动物有67%(4/6)出现轻度肺炎，且无体重下降。肺组织病理学评分和病毒载量显著低于最低鼻腔接种组(100 Pfu)。然而，83%的口腔感染(10/12只仓鼠)从口腔拭子和粪便中检测到病毒脱落的水平与经鼻感染的仓鼠相似。我们的研究结果表明，口服感染SARS-CoV-2可以建立亚临床呼吸道感染，但效率较低。口服接种SARS-CoV-2可以建立金黄地鼠的呼吸道感染模型，口服感染的仓鼠没有体重减轻和疾病体征，口服感染的仓鼠的病毒载量较低，肺部炎症较轻，口服和鼻腔感染的仓鼠具有类似的病毒脱落水平。结果表明，SARS-CoV-2病毒经口接种可引起叙利亚金黄地鼠的亚临床轻度肺炎，但从口腔和粪便中排出的病毒持续时间与经鼻腔感染的金黄地鼠相当。
Severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) is transmitted largely by respiratory droplets or airborne aerosols. Despite being frequently found in the immediate environment and feces of patients, evidence supporting the oral acquisition of SARS-CoV-2 is unavailable. Using the Syrian hamster model, we demonstrate that the severity of pneumonia induced by the intranasal inhalation of SARS-CoV-2 increases with virus inoculum. SARS-CoV-2 retains its infectivity in vitro in simulated human-fed-gastric and fasted-intestinal fluid after 2 h. Oral inoculation with the highest intranasal inoculum (105 PFUs) causes mild pneumonia in 67% (4/6) of the animals, with no weight loss. The lung histopathology score and viral load are significantly lower than those infected by the lowest intranasal inoculum (100 PFUs). However, 83% of the oral infections (10/12 hamsters) have a level of detectable viral shedding from oral swabs and feces similar to that of intranasally infected hamsters. Our findings indicate that the oral acquisition of SARS-CoV-2 can establish subclinical respiratory infection with less efficiency. Oral inoculation of SARS-CoV-2 can establish respiratory infection in hamsters Orally infected hamsters do not have body weight loss and signs of disease Orally infected hamsters have a lower viral load and milder inflammation in lung Orally and intranasally infected hamsters have comparable level of virus shedding Lee et al. demonstrate that SARS-CoV-2 virus can cause subclinical mild pneumonia in Syrian golden hamsters via oral inoculation with less efficiency compared to intranasal inoculation, but virus shedding from oral cavity and feces lasts as long as that in intranasally infected hamsters.
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