Comparison of the effects of ATP, Mg2+, and MgATP on the release of luteinizing hormone-releasing hormone from isolated hypothalamic granules.
Comparison of the effects of ATP, Mg2+, and MgATP on the release of luteinizing hormone-releasing hormone from isolated hypothalamic granules.
复制标题
比较 ATP、Mg2 和 MgATP 对分离的下丘脑颗粒释放黄体生成素释放激素的影响。
DOI：
10.1111/j.1471-4159.1982.tb08664.x
复制
发表时间：
1982
影响因子：
4.7
通讯作者：
Barnea,A
中科院分区：
文献类型：
--
作者：
Burrows,GH;Barnea,A
在体外条件下，观察了ATP、Mg 2+或MgATP对下丘脑颗粒释放促黄体生成素释放激素（LH-RH）的影响。将从成年男性下丘脑分离的颗粒在37°C下在含有0. 15 m-KCl的缓冲（pH 7.8）培养基中孵育。向孵育混合物中添加ATP不会刺激LH-RH的释放。相比之下，添加MgATP刺激LH-RH的释放，释放量比对照高62%。向孵育颗粒中加入Mg 2+也刺激了LH-RH的释放。然而，这种Mg 2+刺激的LH-RH释放的幅度显著低于MgATP刺激的释放（P< 0.01），表明ATP以Mg 2+依赖性方式刺激LH-RH释放。由于MgATP和Mg 2+单独刺激LH-RH释放，我们通过在以下条件之一下孵育颗粒来进一步表征这两种释放过程：在4°C下在含有0. 15 m-KCl的缓冲培养基中孵育或在37°C下在不含KCl的培养基中孵育。在这两种孵育条件下，MgATP刺激的LH-RH释放不明显，而Mg 2+刺激的LH-RH释放明显。基于这些差异，我们提出两个不同的过程可以导致LH-RH从分离的下丘脑颗粒中释放：一个过程涉及ATP和Mg 2+（MgATP），另一个过程仅涉及Mg 2+。
The effect of ATP, Mg2+, or MgATP on the release of luteinizing hormone‐releasing hormone (LH‐RH) from hypothalamic granules was examined underin vitroconditions. Granules, isolated from adult male hypothalami, were incubated at 37°C in a buffered (pH 7.8) medium containing 0.15m‐KCl. The addition of ATP to the incubation mixture did not stimulate the release of LH‐RH. In contrast, the addition of MgATP stimulated the release of LH‐RH, the release being 62% greater than control. The addition of Mg2+to the incubated granules also stimulated the release of LH‐RH. However, the magnitude of this Mg2+‐stimulated release of LH–RH was significantly (P< 0.01) lower than that of the MgATP‐stimulated release, indicating that ATP stimulates LH‐RH release in a Mg2+‐dependent manner. As both MgATP and Mg2+alone stimulated LH‐RH release, we characterized further these two release processes by incubating the granules under one of the following conditions: incubation at 4°C in a buffered medium containing 0.15m‐KCl or incubation at 37°C in a medium that does not contain KCl. Under these two incubation conditions, the MgATP‐stimulated release of LH‐RH was not manifested, whereas the Mg2+‐stimulated release of LH‐RH was manifested. On the basis of these differences, we propose that two different processes can lead to the release of LH‐RH from isolated hypothalamic granules: one process involves ATP and Mg2+(MgATP) and another process involves Mg2+alone.