Detection of membrane associated thioredoxin on human cell lines.
Detection of membrane associated thioredoxin on human cell lines.
复制标题
检测人类细胞系上的膜相关硫氧还蛋白。
DOI：
10.1006/bbrc.1996.6015
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发表时间：
1997
影响因子：
3.1
通讯作者：
D. Fradelizi
中科院分区：
文献类型：
--
作者：
E. Wollman;A. Kahan;D. Fradelizi
硫氧还蛋白 (TRX) 是一种普遍存在的二硫醇氧化还原酶，广泛表达于从原核生物到真核生物的细胞中。人硫氧还蛋白（人 TRX）基因先前在我们实验室克隆，编码在几种造血人类细胞系的培养上清液中发现的 12-kDa 蛋白质。该蛋白质通过非经典途径分泌。分泌酶作为信号可溶性介质的作用已被证明，但对于可能参与细胞/细胞接触和辅助信号功能的膜相关形式的硫氧还蛋白一无所知。因此，我们进行了实验以确定人 TRX 是否也在细胞表面表达。我们在此报告基于使用交叉反应性绵羊抗大肠杆菌 TRX 多克隆抗体对不同人类细胞系（HeLa、U 937、Jurkat、3B6、Daudi 和 Raji）进行间接免疫荧光流式细胞术分析的阳性结果，证明人类 TRX 在人类细胞表面显着表达。
Thioredoxin (TRX) is a ubiquitous dithiol-oxidoreduction enzyme broadly expressed in cells from prokaryote to eukaryote organisms. Human thioredoxin (human TRX) gene, previously cloned in our laboratory, codes for a 12-kDa protein found in the culture supernatant of several hemopoietic human cell lines. This protein is secreted by a nonclassical pathway. The role of the secreted enzyme as a signalling soluble mediator was demonstrated, but nothing is known about a membrane associated form of thioredoxin which could be involved in cell/cell contacts and accessory signal function. Thus, we performed experiments to determine if human TRX is also expressed at the cell surface. We report here positive results based upon indirect immunofluorescence flow cytometric analysis of different human cell lines (HeLa, U 937, Jurkat, 3B6, Daudi and Raji) using a cross reactive sheep anti E. coli TRX polyclonal antibody demonstrating a significant expression of human TRX at the surface of human cells.
DOI：
10.1016/s0021-9258(18)89698-3
发表时间：
1985-01
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J F Grippo;A. Holmgren;W B Pratt
通讯作者：
J F Grippo;A. Holmgren;W B Pratt