Analysis of expressed sequence tags derived from a compatible Mycosphaerella fijiensis-banana interaction
Analysis of expressed sequence tags derived from a compatible Mycosphaerella fijiensis-banana interaction
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DOI：
10.1007/s00299-011-1008-z
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发表时间：
2011-05-01
影响因子：
6.2
通讯作者：
Hofte, Monica
中科院分区：
文献类型：
--
作者：
Portal, Orelvis;Izquierdo, Yovanny;Hofte, Monica
斐济球腔菌（Mycosphaerella fijiensis）是一种半自养真菌，是香蕉和大蕉最严重的叶部病害--黑叶条斑病的病原。分析了M. fijiensis与Musa spp.，构建抑制消减杂交（SSH）cDNA文库以鉴定在宿主和病原体中感染后期诱导的转录物。此外，从与SSH文库相同的mRNA起始材料产生全长cDNA文库。SSH程序是有效的，在确定特定的基因预测参与植物-真菌的相互作用和新的信息，主要是在植物中激活的基因和途径。几个植物基因预测参与合成苯丙烷和解毒化合物，以及病程相关蛋白，可能参与植物对M。斐济人感染。在感染后期，茉莉酸和乙烯信号传导途径显得活跃，这与M.斐济人。定量PCR实验表明，抗真菌基因编码的PR蛋白和GDSL样脂肪酶只瞬时诱导接种后30天（dpi），表明真菌可能是积极抑制植物防御。发现的唯一的真菌基因被诱导37 dpi和编码UDP-葡萄糖焦磷酸化酶，参与海藻糖的生物合成的酶。海藻糖的生物合成可能是由植物抗真菌基因的预先激活引起的，并可能作为一种植物细胞膜损伤的保护剂。
Mycosphaerella fijiensis, a hemibiotrophic fungus, is the causal agent of black leaf streak disease, the most serious foliar disease of bananas and plantains. To analyze the compatible interaction of M. fijiensis with Musa spp., a suppression subtractive hybridization (SSH) cDNA library was constructed to identify transcripts induced at late stages of infection in the host and the pathogen. In addition, a full-length cDNA library was created from the same mRNA starting material as the SSH library. The SSH procedure was effective in identifying specific genes predicted to be involved in plant-fungal interactions and new information was obtained mainly about genes and pathways activated in the plant. Several plant genes predicted to be involved in the synthesis of phenylpropanoids and detoxification compounds were identified, as well as pathogenesis-related proteins that could be involved in the plant response against M. fijiensis infection. At late stages of infection, jasmonic acid and ethylene signaling transduction pathways appear to be active, which corresponds with the necrotrophic life style of M. fijiensis. Quantitative PCR experiments revealed that antifungal genes encoding PR proteins and GDSL-like lipase are only transiently induced 30 days post inoculation (dpi), indicating that the fungus is probably actively repressing plant defense. The only fungal gene found was induced 37 dpi and encodes UDP-glucose pyrophosphorylase, an enzyme involved in the biosynthesis of trehalose. Trehalose biosynthesis was probably induced in response to prior activation of plant antifungal genes and may act as an osmoprotectant against membrane damage.