Heterogeneity in fetal immunocompetence during the second trimester of gestation. Implications for treatment of nonimmune genetic disorders by in utero transplantation.
Heterogeneity in fetal immunocompetence during the second trimester of gestation. Implications for treatment of nonimmune genetic disorders by in utero transplantation.
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妊娠中期胎儿免疫能力的异质性。
DOI：
10.1159/000083900
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
Young,BruceK
中科院分区：
文献类型：
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作者：
Tse,DorisB;Ching,Elbert;Yousefzadeh,Nora;Roque,Hank;Young,BruceK
目的：为了解决的作用，同种异体反应可能发挥和更好地定义窗口组织不相容的干细胞移植在uterate.Subjects，材料和方法：我们研究了9个胎儿血液标本，心脏穿刺在选择性流产在中期妊娠，通过流式细胞仪和体外刺激。6份标本的T细胞在成人范围内。其他标本中T细胞减少，而B细胞则相反升高。这种变异性与胎龄或白细胞组成无关。在有丝分裂发生4 h后，5份标本中有1份的胎儿CD4+和CD8+ T细胞显示出与母体T细胞相似的反应，而其他4份标本显示出减弱的反应（0.3 ± 0.2倍）。这种异质性与胎龄或淋巴细胞亚群分布无关。在体外有丝分裂发生18小时后，来自2个样本的胎儿T细胞显示出与母体T细胞相似的反应（0.8 ± 0.2倍）。尽管如此，一个标本在单向混合淋巴细胞反应与母体细胞相比，其他copyres.Conclusion：我们确定，胎儿免疫能力差异很大，在孕中期和评估主机与供体反应之前，在子宫内移植可能会增强更有利的结果。
Objective:To address the role that alloreactivity may play and better define the window for histoincompatible stem cell transplantation in utero.Subjects, Material and Methods:We studied 9 fetal blood specimens obtained by cardiocentesis during elective abortions in the second trimester by multicolor flow cytometry and in vitro stimulation.Results:Lymphocytes ranged from adult levels (3/9) to >90% leukocytes. Six specimens had T cells within adult range. T cells in the other specimens were reduced, while B cells were conversely elevated. This variability did not correlate with gestational age, or leukocyte composition. Following 4 h of mitogenesis, fetal CD4+ and CD8+ T cells from 1 of 5 specimens showed a response similar to that of maternal T cells, while the other 4 specimens showed a diminished response (0.3 ± 0.2-fold). This heterogeneity did not correlate with gestational age, or lymphocyte subset distribution. Following 18 h of in vitro mitogenesis, fetal T cells from 2 specimens showed a response similar to that of maternal T cells (0.8 ± 0.2-fold). Despite that, one specimen gave a 3-fold greater response in a one-way mixed lymphocyte reaction vs. maternal cells compared to the other specimen.Conclusion:We determine that fetal immunocompetence differs greatly during the second trimester and assessment of host vs. donor reactivity prior to in utero transplantation is likely to potentiate more favorable outcomes.