Retinal organoids: a window into human retinal development.
Retinal organoids: a window into human retinal development.
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DOI：
10.1242/dev.189746
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发表时间：
2020-12-24
期刊：
影响因子：
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通讯作者：
Gonzalez-Cordero A
中科院分区：
文献类型：
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作者：
O'Hara-Wright M;Gonzalez-Cordero A
视网膜的发育和成熟是由一系列相互作用的信号网络精心策划的，这些网络驱动前部发育大脑的形态发生转变。对模式生物的研究继续阐明这些复杂的系列事件。然而，人类视网膜显示出与其他生物体的许多差异，现在对人眼发育的研究受益于干细胞衍生的类器官。视网膜分化方法已经从简单的2D贴壁培养发展到自组织微生理系统。作为发展的模型，这些共同提供了新的见解，以前未探索的人类视网膜的早期发展，并告知我们的知识的关键细胞的命运决定，管理规范的光敏感光细胞。虽然其他视网膜细胞类型的发育轨迹仍然更加难以捉摸，但组学数据集的整理，结合先进的培养方法，将能够在体外模拟人类视网膜发生和视网膜疾病的复杂过程。总结：来源于人类多能干细胞的视网膜类器官系统是微生理系统，为以前未探索的人类视网膜发育提供了新的见解。
Retinal development and maturation are orchestrated by a series of interacting signalling networks that drive the morphogenetic transformation of the anterior developing brain. Studies in model organisms continue to elucidate these complex series of events. However, the human retina shows many differences from that of other organisms and the investigation of human eye development now benefits from stem cell-derived organoids. Retinal differentiation methods have progressed from simple 2D adherent cultures to self-organising micro-physiological systems. As models of development, these have collectively offered new insights into the previously unexplored early development of the human retina and informed our knowledge of the key cell fate decisions that govern the specification of light-sensitive photoreceptors. Although the developmental trajectories of other retinal cell types remain more elusive, the collation of omics datasets, combined with advanced culture methodology, will enable modelling of the intricate process of human retinogenesis and retinal disease in vitro. Summary: Retinal organoid systems derived from human pluripotent stem cells are micro-physiological systems that offer new insights into previously unexplored human retina development.