Lactobacillus oryzae sp. nov., isolated from fermented rice grain (Oryza sativa L. subsp. japonica).
Lactobacillus oryzae sp. nov., isolated from fermented rice grain (Oryza sativa L. subsp. japonica).
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DOI：
10.1099/ijs.0.048918-0
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发表时间：
2013-08
影响因子：
2.8
通讯作者：
M. Tohno;M. Kitahara;T. Irisawa;Hidehiko Inoue;R. Uegaki;M. Ohkuma;K. Tajima
中科院分区：
文献类型：
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作者：
M. Tohno;M. Kitahara;T. Irisawa;Hidehiko Inoue;R. Uegaki;M. Ohkuma;K. Tajima
对从发酵稻谷中分离到的3株类乳杆菌SG 293（T）、SG 296和SG 310进行了分类地位的研究。亚种japonica）在日本进行了调查。这些异型发酵乳酸菌革兰氏染色阳性，杆状，兼性厌氧，非运动，非孢子形成，并没有显示过氧化氢酶活性。菌株SG 293（T）的16 S rRNA基因序列分析表明，模式菌株为马来发酵乳杆菌（98.3%）、气味乳杆菌（96.2%）、相似乳杆菌（96.1%）、泡菜乳杆菌（96.1%）、类干酪乳杆菌（95.9%）和类干酪乳杆菌（95.7%）。pheS和rpoA基因序列的基础上，以及生化和生理特性的其他系统发育分析表明，这三种菌株的属乳杆菌的成员，新的菌株有一个独特的分类地位。主要的细胞脂肪酸是C18：1ω 9 c和C19：1 cyclo 9，10。由于观察到分离物与马来发酵乳杆菌JCM 12497（T）之间的低DNA-DNA杂交值，因此建议将这些未鉴定的分离物分类为乳杆菌属的一个新种，即Lactobacillussp. nov.。模式菌株为SG 293（T）（= JCM 18671（T）= DSM 26518（T））。
The taxonomic position of three Lactobacillus-like micro-organisms (strains SG293(T), SG296 and SG310) isolated from fermented rice grain (Oryza sativa L. subsp. japonica) in Japan was investigated. These heterofermentative lactic acid bacteria were Gram-stain-positive, rod-shaped, facultatively anaerobic, non-motile, non-spore-forming and did not show catalase activity. 16S rRNA gene sequence analysis of strain SG293(T) revealed that the type strains of Lactobacillus malefermentans (98.3 %), Lactobacillus odoratitofui (96.2 %), Lactobacillus similis (96.1 %), Lactobacillus kimchicus (96.1 %), Lactobacillus paracollinoides (95.9 %) and Lactobacillus collinoides (95.7 %) were the closest neighbours. Additional phylogenetic analysis on the basis of pheS and rpoA gene sequences, as well as biochemical and physiological characteristics, indicated that these three strains were members of the genus Lactobacillus and that the novel isolates had a unique taxonomic position. The predominant cellular fatty acids were C18 : 1ω9c and C19 : 1 cyclo 9,10. Because low DNA-DNA hybridization values among the isolates and Lactobacillus malefermentans JCM 12497(T) were observed, it is proposed that these unidentified isolates be classified as a novel species of the genus Lactobacillus, Lactobacillus oryzae sp. nov. The type strain is SG293(T) (= JCM 18671(T) = DSM 26518(T)).