ENHANCED ELASTIN AND FIBRILLIN GENE-EXPRESSION IN CHRONICALLY PHOTODAMAGED SKIN
ENHANCED ELASTIN AND FIBRILLIN GENE-EXPRESSION IN CHRONICALLY PHOTODAMAGED SKIN
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DOI：
10.1111/1523-1747.ep12392693
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发表时间：
1994-08-01
影响因子：
6.5
通讯作者：
UITTO, J
中科院分区：
文献类型：
--
作者：
BERNSTEIN, EF;CHEN, YQ;UITTO, J
皮肤老化包括时间老化和光化损伤，称为光老化。大多数与老化外观相关的形态学变化是由于皮肤的光化性损伤。晒伤皮肤的形态学变化与表皮中具有弹性蛋白染色特征的物质积累有关，称为日光性弹性组织变性。先前的研究已经证明了日光性弹性组织变性区域内存在弹性蛋白;然而，对于导致光老化皮肤中弹性蛋白积聚的机制知之甚少。此外，在日光性弹性组织变性中也发现少量的弹性纤维原纤维成分-。在这项研究中，我们证明增加弹性蛋白mRNA水平在光老化的皮肤，以及增加弹性蛋白和natriin mRNA在皮肤成纤维细胞使用北方杂交，与对照组相比，从同一个人的防晒部位。增加弹性蛋白mRNA水平的结果从转录上调的基因，证明了瞬时转染与人类弹性蛋白启动子/氯霉素乙酰转移酶的构建。mRNA水平升高也与增加弹性蛋白和胶原蛋白沉积在成对的活检标本从光损伤和非阳光照射的皮肤，所示的免疫组化染色。因此，抵消弹性蛋白基因表达的转录激活的方法可能有助于预防与皮肤光老化相关的变化。
Cutaneous aging consists of chronologic aging as well as actinic damage, referred to as photoaging. Most of the morphologic changes associated with an aged appearance result hom actinic damage to the skin. The morphologic changes in sun-damaged skin are associated with accumulation of material having the staining characteristics of elastin, known as solar elastosis, in the superficial dermis. Previous studies have demonstrated the presence of elastin within areas of solar elastosis; however, little is known about the mechanisms leading to elastin accumulation in photoaged skin. In addition, fibrillin, the fibrillar component of elastic fibers, has been found in small amounts in solar elastosis. In this study we demonstrate increased elastin mRNA levels in photoaged skin, as well as increased elastin and fibrillin mRNAs in skin explant-derived fibroblasts using Northern hybridizations, compared with controls from sun-protected sites of the same individual. Increased elastin mRNA levels result from transcriptional upregulation of the gene, as demonstrated by transient transfections with a human elastin promoter/chloramphenicol acetyltransferase construct. Elevated mRNA levels were also correlated with increased elastin and fibrillin deposition in paired biopsy specimens from photodamaged and non-sun-exposed skin, as demonstrated by immunohistochemical staining. Thus, approaches to counteract transcriptional activation of elastin gene expression may be useful in preventing the changes associated with cutaneous photoaging.