OXIDANT STRESS INCREASES HEAT-SHOCK PROTEIN-70 MESSENGER-RNA IN ISOLATED-PERFUSED RAT-HEART
OXIDANT STRESS INCREASES HEAT-SHOCK PROTEIN-70 MESSENGER-RNA IN ISOLATED-PERFUSED RAT-HEART
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DOI：
10.1152/ajpheart.1994.267.6.h2213
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发表时间：
1994-12-01
影响因子：
4.8
通讯作者：
HESS, ML
中科院分区：
文献类型：
--
作者：
KUKREJA, RC;KONTOS, MC;HESS, ML
缺血/再灌注（I/R）和冠状动脉闭塞预处理增加了热休克蛋白70（HSP 70）的表达。由于在I/R过程中产生自由基，我们假设氧化应激可能有助于增加HSP 70的表达。离体大鼠心脏灌注自由基产生系统，如黄嘌呤/黄嘌呤氧化酶（X/XO），照射玫瑰红（RB）产生单线态氧，和H2 O2为15分钟，随后30分钟的恢复期。X/XO、H2 O2和RB灌注后，发展压力和冠状动脉流量显著降低。在I/R期间，发展的压力和冠状动脉流量分别为对照组的60 +/- 8和80 +/-5%，超氧化物歧化酶显著改善。HSP 70 mRNA的表达在X/XO灌注的心脏中增加超过13倍，RB灌注的心脏中增加6- 7倍，H2 O2灌注的心脏中增加超过5倍。与I/R，超过10倍的增加HSP 70 mRNA的观察，这在超氧化物歧化酶的存在下显着下降。这些结果表明，氧化应激直接增加HSP 70 mRNA在大鼠心脏。提示氧自由基可能是I/R诱导HSP 70表达的重要机制之一。
Ischemia/reperfusion (I/R) and preconditioning of the heart by coronary artery occlusions increase expression of heat shock protein 70 (HSP 70). Because free radicals are generated during I/R, we hypothesized that the oxidant stress might contribute to an increased expression of HSP 70. Isolated rat hearts were perfused with free radical-generating systems such as xanthine/ xanthine oxidase (X/XO), irradiated rose bengal (RB) generating singlet oxygen, and H2O2 for 15 min followed by 30 min of recovery period. Significant decrease in developed pressure and coronary flow occurred after perfusion with X/XO, H2O2, and RB. During I/R, the developed pressure and coronary flow were 60 +/- 8 and 80 +/- 5%, respectively, of control, which improved significantly with superoxide dismutase. The expression of HSP 70 mRNA increased over 13-fold in hearts perfused with X/XO, 6- to 7-fold with RB, and over 5-fold with H2O2. With I/R, an over 10-fold increase in HSP 70 mRNA was observed, which decreased significantly in the presence of superoxide dismutase. These results demonstrate that oxidant stress directly increases HSP 70 mRNA in the rat heart. It is concluded that one of the potential mechanisms of expression of HSP 70 by I/R may be oxygen radicals.